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Formation of Double-strand Break in DNA by Spo11

Stable Identifier
R-MMU-912344
Type
Reaction
Species
Mus musculus
Compartment
Locations in the PathwayBrowser
Summation

The gene encoding Spo11 shares sequence similarity to TopoVI, a type II topoisomerase. A Spo11 dimer cleaves both strands of DNA. Each subunit of the dimer remains covalently attached to the 5' end of one strand of DNA via a phosphodiester linkage to a conserved tyrosine residue of Spo11 (Romanienko and Camerini-Otero 2000, Baudat et al. 2000, Mahadevaiah et al. 2001, Zenvirth et al. 2003). In addition to Spo11, work from budding yeast has shown a total of 7 proteins essential for double strand break formation. The mammalian ortholog of Mei4 (S. cerevisiae) as well as a mammalian specific gene called Mei1 are essential to formation of meiotic double strand breaks (Kumar et al.2010, Libby et al. 2003).

Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
endodeoxyribonuclease activity, producing 5'-phosphomonoesters of Spo11 Dimer [nucleoplasm]
Physical Entity
Activity
Orthologous Events