Formation of Double-strand Break in DNA by Spo11

Stable Identifier
R-MMU-912344
Type
Reaction
Species
Mus musculus
Compartment
Summation

The gene encoding Spo11 shares sequence similarity to TopoVI, a type II topoisomerase. A Spo11 dimer cleaves both strands of DNA. Each subunit of the dimer remains covalently attached to the 5' end of one strand of DNA via a phosphodiester linkage to a conserved tyrosine residue of Spo11 (Romanienko and Camerini-Otero 2000, Baudat et al. 2000, Mahadevaiah et al. 2001, Zenvirth et al. 2003). In addition to Spo11, work from budding yeast has shown a total of 7 proteins essential for double strand break formation. The mammalian ortholog of Mei4 (S. cerevisiae) as well as a mammalian specific gene called Mei1 are essential to formation of meiotic double strand breaks (Kumar et al.2010, Libby et al. 2003).

Literature References
PubMed ID Title Journal Year
11242108 Recombinational DNA double-strand breaks in mice precede synapsis

Mahadevaiah, SK, Turner, JM, Baudat, F, Rogakou, EP, de Boer, P, Blanco-Rodriguez, J, Jasin, M, Keeney, S, Bonner, WM, Burgoyne, PS

Nat Genet 2001
14668445 Positional cloning and characterization of Mei1, a vertebrate-specific gene required for normal meiotic chromosome synapsis in mice

Libby, BJ, Reinholdt, LG, Schimenti, JC

Proc Natl Acad Sci U S A 2003
11106738 The mouse Spo11 gene is required for meiotic chromosome synapsis

Romanienko, PJ, Camerini-Otero, RD

Mol Cell 2000
12644952 Mammalian meiosis involves DNA double-strand breaks with 3' overhangs

Zenvirth, D, Richler, C, Bardhan, A, Baudat, F, Barzilai, A, Wahrman, J, Simchen, G

Chromosoma 2003
20551173 Functional conservation of Mei4 for meiotic DNA double-strand break formation from yeasts to mice

Kumar, R, Bourbon, HM, de Massy, B

Genes Dev 2010
11106739 Chromosome synapsis defects and sexually dimorphic meiotic progression in mice lacking Spo11

Baudat, F, Manova, K, Yuen, JP, Jasin, M, Keeney, S

Mol Cell 2000
Participants
Catalyst Activity
Catalyst Activity
Title
endodeoxyribonuclease activity, producing 5'-phosphomonoesters of Spo11 Dimer [nucleoplasm]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed