Fucosylation of Pre-Notch by POFUT1

Stable Identifier
R-NUL-1986584
Type
Reaction [transition]
Species
Cricetulus griseus
Compartment
General
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Fucosylation of Pre-Notch by POFUT1

In the endoplasmic reticulum, Notch receptor precursors are fucosylated on conserved serine and threonine residues in their EGF repeats. The consensus fucosylation site sequence is C2-X(4-5)-S/T-C3, where C2 and C3 are the second and third cysteine residue within the EGF repeat, and X(4-5) is four to five amino acid residues of any type. Only those fucosylation sites that are conserved between human, mouse and rat Notch isoforms are annotated. Fucosylation is performed by the endoplasmic reticulum resident O-fucosyl transferase POFUT1. Fucosylation by POFUT1, initially discovered in Drosophila, is considered to be an essential step in Notch processing and production of a fully functional receptor. Fucosylation of mammalian Notch proteins was studied by exogenously expressing mouse Notch1 in Chinese hamster ovary cell lines.

Literature References
PubMed ID Title Journal Year
11524432 Modification of epidermal growth factor-like repeats with O-fucose. Molecular cloning and expression of a novel GDP-fucose protein O-fucosyltransferase

Wang, Y, Shao, L, Shi, S, Harris, RJ, Spellman, MW, Stanley, P, Haltiwanger, RS

J Biol Chem 2001
15692013 Chaperone activity of protein O-fucosyltransferase 1 promotes notch receptor folding

Okajima, T, Xu, A, Lei, L, Irvine, KD

Science 2005
18347015 Roles of Pofut1 and O-fucose in mammalian Notch signaling

Stahl, M, Uemura, K, Ge, C, Shi, S, Tashima, Y, Stanley, P

J Biol Chem 2008
12486116 Fringe modifies O-fucose on mouse Notch1 at epidermal growth factor-like repeats within the ligand-binding site and the Abruptex region

Shao, L, Moloney, DJ, Haltiwanger, RS

J Biol Chem 2003
21464368 Protein O-fucosyltransferase 1 (Pofut1) regulates lymphoid and myeloid homeostasis through modulation of Notch receptor ligand interactions

Yao, D, Huang, Y, Huang, X, Wang, W, Yan, Q, Wei, L, Xin, W, Gerson, S, Stanley, P, Lowe, JB, Zhou, L

Blood 2011
Participants
Catalyst Activity
Catalyst Activity
Title
peptide-O-fucosyltransferase activity of POFUT1 [endoplasmic reticulum membrane]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed
Created