rDll1 binds NOTCH1 in cis

Stable Identifier
R-NUL-2076711
Type
Reaction [binding]
Species
Homo sapiens
Compartment
General
SVG |   | PPTX  | SBGN
rDll1 binds NOTCH1 in cis

Mammalian cell lines were constructed that allow for independent modulation of the levels of cis- and trans-Delta and quantitative monitoring of the transcriptional response of a Noth reporter gene (Citrine fluorescent protein cDNA fused to a Notch responsive promoter). Cell lines stably expressed recombinant human NOTCH1 and contained a DNA encoding a doxycycline-inducible recombinant rat Dll1 (cis-Dll1). Cell lines were grown on plates containing different concentrations of adsorbed recombinant Dll1 (trans-Dll1). In the absence of cis-Dll1 (no doxycycline added), NOTCH1 activation is proportional to the concentration of trans-Delta. Induction of cis-Dll1 expression blocks NOTCH1 activation, and the response to cis-Dll1 is sharp and switch-like.

Literature References
PubMed ID Title Journal Year
20418862 Cis-interactions between Notch and Delta generate mutually exclusive signalling states

Sprinzak, D, Garcia-Ojalvo, J, Santat, LA, Fontes, ME, LeBon, L, Lakhanpal, A, Elowitz, MB, Anderson, GA

Nature 2010
Participants
Orthologous Events
Authored
Reviewed
Created
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