Smad7 recruits GADD34:PP1CC to phosphorylated TGF-beta receptor complex

Stable Identifier
R-NUL-2167890
Type
Reaction [binding]
Species
Homo sapiens
Compartment
General
SVG |   | PPTX  | SBGN
Smad7 recruits GADD34:PP1CC to phosphorylated TGF-beta receptor complex

Full length mouse Smad7 was used to screen a human chondrocyte cDNA library in a yeast two-hybrid system and PPP1R15A (GADD34) was identified as a binding partner of Smad7. The interaction was confirmed by cotransfection of mouse Smad7 and human PPP1R15A (GADD34) into COS1 cells. The association was positively affected by TGF-beta treatment. Exogenously expressed human TGFB1 receptors and catalytic subunit of PP1, PP1CC, could also be identified in the complex of Smad7 and GADD34. Formation of a complex of TGF-beta receptors, Smad7 and PP1 was dependent on the function of SARA, as shown by expression of dominant negative SARA mutants in COS1 cells. SARA interacts with the catalytic subunit of PP1, likely serving as a membrane anchor for PP1CC (Shi et al. 2004).

Literature References
PubMed ID Title Journal Year
14718519 GADD34-PP1c recruited by Smad7 dephosphorylates TGFbeta type I receptor

Shi, X, Sun, C, Cao, X, Yao, D, He, B, Shi, W, Xiong, W

J Cell Biol 2004
Participants
Orthologous Events
Authored
Reviewed
Created
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