Phosphorylation of Gorasp1, Golga2 and RAB1A by CDK1:CCNB

Stable Identifier
R-NUL-2422970
Type
Reaction [transition]
Species
Homo sapiens
Compartment
General
SVG |   | PPTX  | SBGN
Phosphorylation of Gorasp1, Golga2 and RAB1A by CDK1:CCNB

The existence of Gorasp1:Golga2:Uso1:Rab1:GTP complex was established by co-immunoprecipitation of exogenously expressed human RAB1A (identical sequence to rat Rab1A) from normal rat kidney cells, NRKs (Moyer et al. 2001). It was also shown that human RAB1B binds human GM130 (Weide et al. 2001). In mitotic prophase, human CDK1 (CDC2) in complex with either CCNB1 (cyclin B1) or CCNB2 (cyclin B2), as both CCNB1 and CCNB2 can localize to Golgi (Jackman et al. 1995, Draviam et al. 2001), was shown to phosphorylate recombinant rat Gorasp1 and Golga2, as well as RAB1A (Bailly et al. 1991, Lowe et al. 1998, Preisinger et al. 2005) . Phosphorylation of Golga2 and RAB1A impairs their association with Uso1 (Lowe et al. 1998, Seeman et al. 2000, Moyer et al. 2001).

Literature References
PubMed ID Title Journal Year
1902553 Phosphorylation of two small GTP-binding proteins of the Rab family by p34cdc2

Bailly, E, McCaffrey, M, Touchot, N, Zahraoui, A, Goud, B, Bornens, M

Nature 1991
11285137 Rab1 interaction with a GM130 effector complex regulates COPII vesicle cis--Golgi tethering

Moyer, BD, Allan, BB, Balch, WE

Traffic 2001
7737117 Human cyclins B1 and B2 are localized to strikingly different structures: B1 to microtubules, B2 primarily to the Golgi apparatus

Jackman, M, Firth, M, Pines, J

EMBO J 1995
15678101 Plk1 docking to GRASP65 phosphorylated by Cdk1 suggests a mechanism for Golgi checkpoint signalling

Preisinger, C, Korner, R, Wind, M, Lehmann, WD, Kopajtich, R, Barr, FA

EMBO J 2005
11238451 The localization of human cyclins B1 and B2 determines CDK1 substrate specificity and neither enzyme requires MEK to disassemble the Golgi apparatus

Draviam, VM, Orrechia, S, Lowe, M, Pardi, R, Pines, J

J Cell Biol 2001
9753325 Cdc2 kinase directly phosphorylates the cis-Golgi matrix protein GM130 and is required for Golgi fragmentation in mitosis

Lowe, M, Rabouille, C, Nakamura, N, Watson, R, Jackman, M, Jämsä, E, Rahman, D, Pappin, DJC, Warren, G

Cell 1998
11306556 The Golgi matrix protein GM130: a specific interacting partner of the small GTPase rab1b

Weide, T, Bayer, M, Köster, M, Siebrasse, JP, Peters, R, Barnekow, A

EMBO Rep. 2001
10679020 The role of the tethering proteins p115 and GM130 in transport through the Golgi apparatus in vivo

Seemann, J, Jokitalo, EJ, Warren, G

Mol. Biol. Cell 2000
Participants
Catalyst Activity
Catalyst Activity
Title
cyclin-dependent protein serine/threonine kinase activity of CCNB1,CCNB2:p-T161-CDK1 [cytosol]
Physical Entity
Activity
Orthologous Events
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Reviewed
Created