TOM40:TOM70 complex translocates proteins from the cytosol to the mitochondrial intermembrane space

Stable Identifier
R-SCE-1252255
Type
Reaction [transition]
Species
Saccharomyces cerevisiae
Compartment
cytosol, mitochondrial intermembrane space, mitochondrial outer membrane
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
TOM40:TOM70 complex translocates proteins from the cytosol to the mitochondrial intermembrane space

The TOM40:TOM70 Complex transports precursor proteins from the cytosol, across the outer membrane of the mitochondrion, and into the intermembrane space from where they may be targeted to all locations within the mitochondrion. TOM40, TOM22, TOM5, TOM6, and TOM7 form the general import pore across the membrane (Dekker et al. 1998). TOM20 and TOM22 interact with presequences on mitochondrial precursors (Haucke et al. 1995, Brix et al. 1997, Meisinger et al. 2001, Yamano et al. 2008). TOM70 interacts with hydrophobic sequences in mature internal regions of mitochondrial proteins (Schlossman et al. 1994, Brix et al. 1997, Yamamoto et al. 2009).
Substrates of the TOM40:TOM70 complex include ATP1 (Yamamoto et al. 2009), ATP2 (Yamamoto et al. 2009), ATP9 (Hines et al. 1990), TOM40 (Yamamoto et al. 2009), SSC1 (Yamamoto et al. 2009), CIT1 (Yamamoto et al. 2009), ACO1 (Yamamoto et al. 2009), IDH1 (Yamamoto et al. 2009), BCS1 (Stan et al. 2003), CYT1 (Diekert et al. 2001), TIM54 (Kurz et al. 1999), TIM22 (Kurz et al. 1999), AAC (Sollner et al. 1990, Kurz et al. 1990, Kubrich et al. 1998, Endres et al. 1999, Truscott et al. 2002, Vasiljev et al. 2004), HSP60 (Yamano et al. 2008), CYB2 (Krayl et al. 2007). Some proteins such as Aco1, Atp1, Cit1, Idh1, and Atp2 contain both presequences that interact with TOM20 and mature regions that interact with TOM70 (Yamamoto et al. 2009).

Literature References
PubMed ID Title Journal Year
11598006 Apocytochrome c requires the TOM complex for translocation across the mitochondrial outer membrane

Diekert, K, de Kroon, AI, Ahting, U, Niggemeyer, B, Neupert, W, de Kruijff, B, Lill, R

EMBO J 2001
7890675 The yeast mitochondrial protein import receptor Mas20p binds precursor proteins through electrostatic interaction with the positively charged presequence

Haucke, V, Lithgow, T, Rospert, S, Hahne, K, Schatz, G

J Biol Chem 1995
21896724 In vivo protein-interaction mapping of a mitochondrial translocator protein Tom22 at work

Shiota, T, Mabuchi, H, Tanaka-Yamano, S, Yamano, K, Endo, T

Proc Natl Acad Sci U S A 2011
8163488 Specific recognition of mitochondrial preproteins by the cytosolic domain of the import receptor MOM72

Schlossmann, J, Dietmeier, K, Pfanner, N, Neupert, W

J Biol Chem 1994
12391147 Mitochondrial import of the ADP/ATP carrier: the essential TIM complex of the intermembrane space is required for precursor release from the TOM complex

Truscott, KN, Wiedemann, N, Rehling, P, Müller, H, Meisinger, C, Pfanner, N, Guiard, B

Mol Cell Biol 2002
17074805 A cooperative action of the ATP-dependent import motor complex and the inner membrane potential drives mitochondrial preprotein import

Krayl, M, Lim, JH, Martin, F, Guiard, B, Voos, W

Mol Cell Biol 2007
10369662 Transport of the ADP/ATP carrier of mitochondria from the TOM complex to the TIM22.54 complex

Endres, M, Neupert, W, Brunner, M

EMBO J 1999
19767391 Roles of Tom70 in import of presequence-containing mitochondrial proteins

Yamamoto, H, Fukui, K, Takahashi, H, Kitamura, S, Shiota, T, Terao, K, Uchida, M, Esaki, M, Nishikawa, S, Yoshihisa, T, Yamano, K, Endo, T

J Biol Chem 2009
2163763 A mitochondrial import receptor for the ADP/ATP carrier

Söllner, T, Pfaller, R, Griffiths, G, Pfanner, N, Neupert, W

Cell 1990
9774667 Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex

Dekker, PJ, Ryan, MT, Brix, J, Müller, H, Honlinger, A, Pfanner, N

Mol Cell Biol 1998
10397776 Biogenesis of Tim proteins of the mitochondrial carrier import pathway: differential targeting mechanisms and crossing over with the main import pathway

Kurz, M, Martin, H, Rassow, J, Pfanner, N, Ryan, MT

Mol Biol Cell 1999
9632701 The import route of ADP/ATP carrier into mitochondria separates from the general import pathway of cleavable preproteins at the trans side of the outer membrane

Kübrich, M, Rassow, J, Voos, W, Pfanner, N, Honlinger, A

J Biol Chem 1998
14668492 Reconstituted TOM core complex and Tim9/Tim10 complex of mitochondria are sufficient for translocation of the ADP/ATP carrier across membranes

Vasiljev, A, Ahting, U, Nargang, FE, Go, NE, Habib, SJ, Kozany, C, Panneels, V, Sinning, I, Prokisch, H, Neupert, W, Nussberger, S, Rapaport, D

Mol Biol Cell 2004
11259583 Protein import channel of the outer mitochondrial membrane: a highly stable Tom40-Tom22 core structure differentially interacts with preproteins, small tom proteins, and import receptors

Meisinger, C, Ryan, MT, Hill, K, Model, K, Lim, JH, Sickmann, A, Müller, H, Meyer, HE, Wagner, R, Pfanner, N

Mol Cell Biol 2001
9252394 Differential recognition of preproteins by the purified cytosolic domains of the mitochondrial import receptors Tom20, Tom22, and Tom70

Brix, J, Dietmeier, K, Pfanner, N

J Biol Chem 1997
18063580 Tom20 and Tom22 share the common signal recognition pathway in mitochondrial protein import

Yamano, K, Yatsukawa, Y, Esaki, M, Hobbs, AE, Jensen, RE, Endo, T

J Biol Chem 2008
12640110 Mitochondrial protein import: recognition of internal import signals of BCS1 by the TOM complex

Stan, T, Brix, J, Schneider-Mergener, J, Pfanner, N, Neupert, W, Rapaport, D

Mol Cell Biol 2003
2170106 Protein import into yeast mitochondria is accelerated by the outer membrane protein MAS70

Hines, V, Brandt, A, Griffiths, G, Horstmann, H, Brutsch, H, Schatz, G

EMBO J 1990
Participants
Input
Output
Catalyst Activity
Catalyst Activity
Title
protein transmembrane transporter activity of TOM complex [mitochondrial outer membrane]
Physical Entity
TOM complex [mitochondrial outer membrane]
Activity
protein transmembrane transporter activity (0008320)
Orthologous Events
TOMM40 complex translocates proteins from the cytosol to the mitochondrial intermembrane space (Homo sapiens)
Authored
May, B  (2011-04-22)
Reviewed
D'Eustachio, P  (2011-11-02)
Endo, T  (2012-02-12)
Created
May, B  (2011-04-27)
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