EMG1 of the SSU processome methylates pseudouridine-1191 of 18S rRNA yielding N(1)-methylpseudouridine-1191

Stable Identifier
R-SCE-6790936
Type
Reaction [transition]
Species
Saccharomyces cerevisiae
Compartment
nucleoplasm
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
EMG1 of the SSU processome methylates pseudouridine-1191 of 18S rRNA yielding N(1)-methylpseudouridine-1191

EMG1 (NEP1) methylates a pseudouridine residue in precursor rRNA (pre-rRNA) to yield N(1)-methylpseudouridine (Meyer et al. 2011). Following further modification and nucleolytic processing, the N(1)-methylpseudouridine residue becomes N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine-1191 of the 18S rRNA. EMG1 recognizes the nucleotide to be methylated by base-specific interactions with RNA (Thomas et al. 2011). EMG1 is a component of the small subunit processome (SSU processome) a large complex of proteins that binds the 5' region of pre-rRNA, processes and modifies the 18S rRNA, and assists the assembly of the small ribosomal subunit (Bernstein et al. 2004).

Literature References
PubMed ID Title Journal Year
21087996 Structural insight into the functional mechanism of Nep1/Emg1 N1-specific pseudouridine methyltransferase in ribosome biogenesis

Thomas, SR, Keller, CA, Szyk, A, Cannon, JR, Laronde-Leblanc, NA

Nucleic Acids Res. 2011
15590835 The small-subunit processome is a ribosome assembly intermediate

Bernstein, KA, Gallagher, JE, Mitchell, BM, Granneman, S, Baserga, SJ

Eukaryotic Cell 2004
20972225 The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Ψ1191 in yeast 18S rRNA

Meyer, B, Wurm, JP, Kötter, P, Leisegang, MS, Schilling, V, Buchhaupt, M, Held, M, Bahr, U, Karas, M, Heckel, A, Bohnsack, MT, Wöhnert, J, Entian, KD

Nucleic Acids Res. 2011
Participants
Input
Output
Event Information
Go Biological Process
rRNA methylation (0031167)
Catalyst Activity
Catalyst Activity
Title
rRNA (pseudouridine) methyltransferase activity of SSU Processome [nucleoplasm]
Physical Entity
SSU Processome [nucleoplasm]
Activity
rRNA (pseudouridine) methyltransferase activity (0070037)
Orthologous Events
EMG1 of the SSU processome methylates pseudouridine-1248 of 18S rRNA yielding N(1)-methylpseudouridine-1248 (Homo sapiens)
Authored
May, B  (2015-08-15)
Reviewed
Vincent, NG  (2016-01-30)
Created
May, B  (2015-08-15)