Box C/D snoRNP methylates ribonucleotides in pre-rRNA

Stable Identifier
R-SCE-6793739
Type
Reaction
Species
Saccharomyces cerevisiae
Compartment
Summation

The NOP1 component of box C/D small nucleolar ribonucleoprotein complexes (snoRNPs) methylates the 2' position of the ribose moiety of nucleotides in precursor ribosomal RNA (pre-rRNA) (Kiss-Laszlo et al. 1996, Galardi et al. 2002). Box C/D snoRNPs contain NOP1 (homologous to Fibrillarin in humans), NOP56, NOP58 (NOP5), SNU13 (homologous to 15.5K, NHP2L1 in humans), and a box C/D snoRNA, which guides the snoRNP to the site of methylation by base pairing with 10-21 nucleotides of the pre-rRNA (Schimmang et al. 1989, Kiss-Laszlo et al. 1998, Wu et al. 1998, Lafontaine and Tollervey 1999, Lafontaine and Tollervey 2000, Watkins et al. 2000, Galardi et al. 2002).

Literature References
PubMed ID Title Journal Year
9632712 Nop5p is a small nucleolar ribonucleoprotein component required for pre-18 S rRNA processing in yeast

Wu, P, Brockenbrough, JS, Metcalfe, AC, Chen, S, Aris, JP

J. Biol. Chem. 1998
10094313 Nop58p is a common component of the box C+D snoRNPs that is required for snoRNA stability

Lafontaine, DL, Tollervey, D

RNA 1999
9372940 Nucleolar KKE/D repeat proteins Nop56p and Nop58p interact with Nop1p and are required for ribosome biogenesis

Gautier, T, Bergčs, T, Tollervey, D, Hurt, E

Mol. Cell. Biol. 1997
11081632 A common core RNP structure shared between the small nucleoar box C/D RNPs and the spliceosomal U4 snRNP

Watkins, NJ, Ségault, V, Charpentier, B, Nottrott, S, Fabrizio, P, Bachi, A, Wilm, M, Rosbash, M, Branlant, C, Lührmann, R

Cell 2000
8674114 Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs

Kiss-László, Z, Henry, Y, Bachellerie, JP, Caizergues-Ferrer, M, Kiss, T

Cell 1996
2686980 A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability

Schimmang, T, Tollervey, D, Kern, H, Frank, R, Hurt, EC

EMBO J. 1989
10733567 Synthesis and assembly of the box C+D small nucleolar RNPs

Lafontaine, DL, Tollervey, D

Mol. Cell. Biol. 2000
9451004 Sequence and structural elements of methylation guide snoRNAs essential for site-specific ribose methylation of pre-rRNA

Kiss-László, Z, Henry, Y, Kiss, T

EMBO J. 1998
12215523 Purified box C/D snoRNPs are able to reproduce site-specific 2'-O-methylation of target RNA in vitro

Galardi, S, Fatica, A, Bachi, A, Scaloni, A, Presutti, C, Bozzoni, I

Mol. Cell. Biol. 2002
Participants
Event Information
Go Biological Process
Catalyst Activity
Catalyst Activity
Title
O-methyltransferase activity of Box C/D snoRNP [nucleoplasm]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed