Reactome: A Curated Pathway Database

Removal of SPO11 and Resection of 5' Ends of DNA (yeast)

Stable Identifier
R-SCE-981784
Type
Reaction [BlackBoxEvent]
Species
Saccharomyces cerevisiae
Compartment
Summation

SPO11 forms a dimer and each subunit cleaves a single strand of DNA, thus creating a double-strand break. After cleaving DNA, a SPO11 subunit remains covalently attached to each 5' end via a tyrosine residue. SPO11 is removed from the DNA by cleavage of single strands 3' to the attached SPO11. The products are a resected 5' end (protruding 3' overhang) and a covalent complex of SPO11 with an oligonucleotide.

Literature References
Participants
This entity is regulated by:
Title Physical Entity Activity
3'-5'-exodeoxyribonuclease activity of MRX:SAE2 Complex [nucleoplasm] MRX:SAE2 Complex [nucleoplasm] 3'-5'-exodeoxyribonuclease activity (0008296)
endodeoxyribonuclease activity of MRX:SAE2 Complex [nucleoplasm] MRX:SAE2 Complex [nucleoplasm] endodeoxyribonuclease activity (0004520)
Orthologous Events