Xenopus Mcm8 mediated fork unwinding

Stable Identifier
R-XLA-169440
Type
Reaction [transition]
Species
Xenopus laevis
Compartment
nucleoplasm
ReviewStatus
5/5
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
Xenopus Mcm8 mediated fork unwinding
Mcm8 is related to the Mcm2-7 helicase and is found in vertebrates but not in C. elegans or the yeasts. Studies in Xenopus egg extracts showed that Mcm8 does not associate with the soluble Mcm2-7 complex and binds chromatin upon initiation of DNA synthesis. Mcm8 depletion does not affect replication licensing or Mcm3 loading but slows down DNA synthesis and reduces chromatin recruitment of Rpa34 and DNA polymerase-alpha. Recombinant Mcm8 has ATPase and helicase activities.
Literature References
PubMed ID Title Journal Year
15707891 MCM8 is an MCM2-7-related protein that functions as a DNA helicase during replication elongation and not initiation

Maiorano, D, Cuvier, O, Danis, E, Mechali, M

Cell 2005
Participants
Input
Output
Catalyst Activity

helicase activity of xMcm8 [nucleoplasm]

Physical Entity
xMcm8 [nucleoplasm] (Xenopus laevis)
Activity
helicase activity (GO:0004386)
Orthologous Events
MCM8 mediated fork unwinding (Homo sapiens)
Authored
Tye, BK  (2005-11-29)
Created
Gopinathrao, G  (2005-11-29)
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