Cytochrome P450 8B1 (CYP8B1, sterol 12-alpha- hydroxylase) has a broad substrate specificity including a number of 7-alpha- hydroxylated C27 steroids. It is also involved in bile acid synthesis and is responsible for the balance between the formation of cholic acid and chenodeoxycholic acid (Gafvels et al. 1999).
Sterol 12alpha hydroxylase (CYP8B1), an enzyme associated with the endoplasmic reticulum membrane, catalyses the 12-alpha-hydroxylation of 7-Alpha-hydroxycholest-4-en-3-one (4CHOL7aOLONE) to 7-alpha,12-alpha-dihydroxycholest-4-en-3-one (4CHOL7a,12aDONE). While the human gene has been cloned (Gafvels et al. 1999), its protein product has not been characterised, and the enzymatic properties of human CYP8B1 protein are inferred from those of its well-characterised rabbit homolog (Ishida et al. 1992).
Sterol 12alpha hydroxylase (CYP8B1), associated with the endoplasmic reticulum membrane, catalyses the 12-alpha-hydroxylation of 4-Cholesten-7alpha,27-diol-3-one (4CHOL7a,27DONE) to 4-Cholesten-7alpha,12alpha,27-triol-3-one. While the human gene has been cloned (Gafvels et al. 1999), its protein product has not been characterised, and the enzymatic properties of human CYP8B1 protein are inferred from those of its well-characterised rabbit homolog (Ishida et al. 1992).
Sterol 12alpha hydroxylase (CYP8B1) catalyses the 12-alpha-hydroxylation of 4-cholesten-7alpha,24(S)-diol-3-one (4CHOL7a,24(S)DONE) to 4-cholesten-7-alpha,12-alpha,24(S)-triol-3-one (4CHOL7a,12a,24(S)TONE). While the human gene has been cloned (Gafvels et al. 1999), its protein product has not been characterised, and the enzymatic properties of human CYP8B1 protein are inferred from those of its well-characterised rabbit homolog (Ishida et al. 1992).
This CandidateSet contains sequences identified by William Pearson's analysis of Reactome catalyst entities. Catalyst entity sequences were used to identify analagous sequences that shared overall homology and active site homology. Sequences in this Candidate set were identified in an April 24, 2012 analysis. Further candidates were identified by Ralf Stephan in a July 15, 2023 analysis.
This CandidateSet contains sequences identified by William Pearson's analysis of Reactome catalyst entities. Catalyst entity sequences were used to identify analagous sequences that shared overall homology and active site homology. Sequences in this Candidate set were identified in an April 24, 2012 analysis. Further candidates were identified by Ralf Stephan in a July 15, 2023 analysis.