5'-nucleotidase (NT5E) associated with the plasma membrane catalyzes the reactions of extracellular CMP, TMP, or UMP with H2O to yield the corresponding nucleoside and orthophosphate. The active enzyme is a glycolipid-anchored dimer (Misumi et al. 1990; Thompson et al. 1987; Zimmerman 1992)
5'-nucleotidase (NT5E, CD73) is able to hydrolyse extracellular nucleotides into membrane permeable nucleosides. It displays a broad specificity, acting on mono- or di-nucleotide nicotinamides and different adenosine phosphates, with maximal activity on 5'-adenosine monophosphate. Human NT5E can hydrolyse both NAD+ and NMN, suggesting a role in NAD metabolism (Garavaglia et al. 2012). NT5E is a glycolipid-anchored plasma membrane enzyme (Misumi et al. 1990) that is active in dimeric form and requires one zinc ion per subunit (Zimmermann 1992).
5'-nucleotidase (NT5E, CD73) is able to hydrolyse extracellular nucleotides into membrane permeable nucleosides. It displays a broad specificity, acting on mono- or di-nucleotide nicotinamides and different adenosine phosphates, with maximal activity on 5'-adenosine monophosphate. Human NT5E can hydrolyse both NAD+ and NMN, suggesting a role in NAD metabolism (Garavaglia et al. 2012). NT5E is a glycolipid-anchored plasma membrane enzyme (Misumi et al. 1990) that is active in dimeric form and requires one zinc ion per subunit (Zimmermann 1992).
5'-nucleotidase (NT5E) associated with the plasma membrane catalyzes the reactions of extracellular AMP, dAMP, GMP, or IMP with H2O to yield the corresponding nucleoside and orthophosphate. The active enzyme is a glycolipid-anchored dimer (Misumi et al. 1990; Thompson et al. 1987; Zimmerman 1992)