Search results for TIAM2
Showing 6 results out of 6
Protein (1 results from a total of 1)
Identifier: R-HSA-561165
Species:
Homo sapiens
Compartment:
cytosol
Primary external reference: UniProt: TIAM2:
Q8IVF5
DNA Sequence (1 results from a total of 1)
Identifier: R-HSA-5649926
Species:
Homo sapiens
Compartment:
nucleoplasm
Primary external reference: ENSEMBL:
ENSEMBL:ENSG00000146426
Reaction (4 results from a total of 4)
Identifier: R-HSA-1989768
Species:
Homo sapiens
Compartment:
nucleoplasm,
cytosol
The TIAM2 gene is transcribed to yield mRNA and the mRNA is translated to yield protein.
Identifier: R-HSA-8980691
Species:
Homo sapiens
Compartment:
cytosol,
plasma membrane
The following guanine nucleotide exchange factors (GEFs) were shown to bind RHOA and catalyze GDP to GTP exchange on RHOA, resulting in formation of the active RHOA:GTP complex (the high throughput study by Bagci et al. 2020 looked at preferential binding of GEFs to nucleotide-free RHOA mutant compared to wild type RHOA but did not examine guanine nucleotide exchange):
AKAP13 (Zheng et al. 1995; Müller et al. 2020; supported by Bagci et al. 2020)
ARHGEF1 (Hart et al. 1996; Jaiswal et al. 2011; Jaiswal et al. 2013; Toffali et al. 2017; Müller et al. 2020; supported by Bagci et al. 2020)
ARHGEF2 (Krendel et al. 2002; Müller et al. 2020; supported by Bagci et al. 2020)
ARHGEF3 (Arthur et al. 2002; Sloan et al. 2012; Müller et al. 2020)
ARHGEF5 (Xie et al. 2005; Wang et al. 2009; supported by Bagci et al. 2020)
ARHGEF10 (Mohl et al. 2006; Aoki et al. 2009; Müller et al. 2020)
ARHGEF11 (Rümenapp et al. 1999; Jaiswal et al. 2011; Jaiswal et al. 2013; Müller et al. 2020; supported by Bagci et al. 2020)
ARHGEF12 (Reuther et al. 2001; Jaiswal et al. 2011; Jaiswal et al. 2013; Müller et al. 2020; supported by Bagci et al. 2020)
ARHGEF15 (Fukushima et al. 2016; Müller et al. 2020)
ARHGEF17 (Rümenapp et al. 2002; Mitin et al. 2012; Müller et al. 2020; supported by Bagci et al. 2020)
ARHGEF19 (Wang et al. 2004; Müller et al. 2020)
ARHGEF25 (Sloan et al. 2012; Müller et al. 2020)
ARHGEF28 (van Horck et al. 2001; Jaiswal et al. 2011; Jaiswal et al. 2013; Müller et al. 2020)
ECT2 (Tatsumoto et al. 1999; Müller et al. 2020; supported by Bagci et al. 2020)
MCF2 (Sloan et al. 2012; Jaiswal et al. 2013; Müller et al. 2020)
NET1 (Alberts and Treisman 1998; Müller et al. 2020)
NGEF (Zhang et al. 2007; Müller et al. 2020; supported by Bagci et al. 2020)
OBSCN (Ford Speelman et al. 2009)
PLEKHG5 (de Toledo et al. 2001; Liu and Horowitz 2006; Müller et al. 2020)
PLEKHG6 (Müller et al. 2020)
RASGRF2 (Müller et al. 2020)
TRIO (TRIO and KALRN possess two GEF domains, the N-terminal GEF1 and the C-terminal GEF2; Debant et al. 1996 demonstrated activation of RHOA by the C-terminal GEF2 domain of TRIO, while Peurois et al. 2017 demonstrated activation of RHOA by the N-terminal GEF1 domain of TRIO in the presence of membranes, Williams et al. 2007 showed that in C-elegans GNAQ-mediated activation of TRIO results in RHOA activation that drived locomotion and egg laying, and Bagci et al. 2020 reported binding of inactive RHOA mutant to the full-length TRIO; Debant et al. 1996 and Jaiswal et al. 2013 showed that the N-terminal GEF1 domain of TRIO does not activate RHOA; Müller et al. 2020 detected no activation of RHOA in the presence of full-length TRIO, but in this study HEK293 cells were used, which were previously shown by Williams et al. 2007 to have a low TRIO-activation ability downstream of GNAQ)
VAV1 (Heo et al. 2005; Müller et al. 2020)
VAV2 (Heo et al. 2005; Sloan et al. 2012; Schuebel et al. 1998; Jaiswal et al. 2013; Müller et al. 2020; supported by Bagci et al. 2020)
VAV3 (Movilla and Bustelo 1999, Sachdev et al. 2002; Müller et al. 2020)
They are annotated as RHOA GEF set members.
The following GEFs were shown to bind and activate RHOA in some but not all studies, or have not been sufficiently studied, and are annotated as candidate RHOA GEFs:
ABR (Chuang et al. 1995, Manser et al. 1998: activation of RHOA; Müller et al. 2020: no activation of RHOA)
ARHGEF4 (Müller et al. 2020: activation of RHOA; Gotthardt and Ahmadian 2007, Anderson and Hamann 2012, Jaiswal et al. 2013: no activation of RHOA)
ARHGEF7 (Manser et al. 1998: activation of RHOA; Jones and Katan 2007, Müller et al. 2020: no activation of RHOA; Bagci et al. 2020: no binding to RHOA)
ARHGEF10L (Winkler et al. 2005: activation of RHOA; Müller et al. 2020: no activation of RHOA)
ARHGEF18 (Niu et al. 2003, Blomquist et al. 2000: activation of RHOA; Müller et al. 2020: no activation of RHOA)
ARHGEF40 (Curtis et al. 2004: activation of RHOA; Müller et al. 2020: no activation of RHOA; Bagci et al. 2020: no binding to RHOA)
BCR (Chuang et al. 1995: activation of RHOA; Bagci et al. 2020: binding to RHOA; Müller et al. 2020: no activation of RHOA)
DEF6 (Mavrakis et al. 2004: probable GEF, in vitro GEF activity not tested)
DOCK2 (Toffali et al. 2017: inconclusive; Müller et al. 2020: no activation of RHOA)
FARP1 (Koyano et al. 2001: activation of RHOA; Müller et al. 2020: no activation of RHOA; Bagci et al. 2020: inconclusive binding to RHOA)
KALRN (Penzes et al. 2001: the C-terminal GEF2 domain of KALRN binds RHOA and activates it, although activation was not demonstrated in vitro, while the N-terminal GEF1 domain of KALRN does not bind to RHOA; Wu et al. 2013: no effect on RHOA activity in mice with heterozygous Kalrn gene knockout; Müller et al. 2020: no activation of RHOA by full-length KALRN)
MCF2L (Whitehead et al. 1999, Sloan et al. 2012, Jaiswal et al. 2013, Müller et al. 2020: activation of RHOA; Bagci et al. 2020: no binding to RHOA)
PLEKHG3 (Bagci et al. 2020: binding to RHOA, RHOA activation not tested; Müller et al. 2020: no activation of RHOA)
PLEKHG4 (Gupta et al. 2013: activation of RHOA; Müller et al. 2020: no activation of RHOA; Bagci et al. 2020: no binding to RHOA)
PREX1 (Jaiswal et al. 2013: activation of RHOA; Marei et al. 2016, Müller et al. 2020: no activation of RHOA)
PREX2 (Müller et al. 2020: activation of RHOA; Joseph and Norris 2005: no activation of RHOA)
TIAM1 (Michiels et al. 1995: activation of RHOA; Jaiswal et al. 2013, Müller et al. 2020: no activation of RHOA; Bagci et al. 2020: no binding to RHOA)
The following GEFs do not act on RHOA or have been reported to not bind to RHOA, as indicated, without testing for activity:
ALS2 (Müller et al. 2020)
ARHGEF6 (Ramakers et al. 2012; Müller et al. 2020)
ARHGEF9 (Reid et al. 1999; Jaiswal et al. 2013; Müller et al. 2020)
ARHGEF16 (Hiramoto Yamaki et al. 2010; Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
ARHGEF26 (Ellerbroek et al. 2004; Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
ARHGEF39 (Müller et al. 2020)
DNMBP (Jaiswal et al. 2013; Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
DOCK1 (Côté and Vuori 2002; Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
DOCK3 (Müller et al. 2020)
DOCK4 (Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
DOCK5 (Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
DOCK6 (Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
DOCK7 (Müller et al. 2020; no binding to RHOA byBagci et al. 2020)
DOCK8 (Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
DOCK9 (Ruiz Lafuente et al. 2015; Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
DOCK10 (Ruiz Lafuente et al. 2015; Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
DOCK11 (Ruiz Lafuente et al. 2015; Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
ECT2L (Müller et al. 2020)
FARP2 (Kubo et al. 2002; Müller et al. 2020);
FGD1 (Olson et al. 1996; Müller et al. 2020)
FGD2 (Huber et al. 2008; Müller et al. 2020)
FGD3 (Müller et al. 2020)
FGD5 (Müller et al. 2020)
FGD6 (Müller et al. 2020)
FGD4 (Umikawa et al. 1999; Müller et al. 2020)
GNA13 (Teo et al. 2016)
ITSN1 (Hussain et al. 2001; Jaiswal et al. 2013; Müller et al. 2020)
ITSN2 (Müller et al. 2020)
MCF2L2 (Müller et al. 2020)
PLEKHG1 (Abiko et al. 2015; Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
PLEKHG2 (Ueda et al. 2008; Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
PLEKHG4B (Müller et al. 2020)
SOS1 (Nimnual et al. 1998; Müller et al. 2020)
SOS2 (Nimnual et al. 1998; Müller et al. 2020)
SPATA13 (Hamann et al. 2007; Kawasaki et al. 2007; Bristow et al. 2009; Müller et al. 2020)
SWAP70 (Ocana Morgner et al. 2009; no binding to RHOA by Bagci et al. 2020)
TIAM2 (Müller et al. 2020)
AKAP13 (Zheng et al. 1995; Müller et al. 2020; supported by Bagci et al. 2020)
ARHGEF1 (Hart et al. 1996; Jaiswal et al. 2011; Jaiswal et al. 2013; Toffali et al. 2017; Müller et al. 2020; supported by Bagci et al. 2020)
ARHGEF2 (Krendel et al. 2002; Müller et al. 2020; supported by Bagci et al. 2020)
ARHGEF3 (Arthur et al. 2002; Sloan et al. 2012; Müller et al. 2020)
ARHGEF5 (Xie et al. 2005; Wang et al. 2009; supported by Bagci et al. 2020)
ARHGEF10 (Mohl et al. 2006; Aoki et al. 2009; Müller et al. 2020)
ARHGEF11 (Rümenapp et al. 1999; Jaiswal et al. 2011; Jaiswal et al. 2013; Müller et al. 2020; supported by Bagci et al. 2020)
ARHGEF12 (Reuther et al. 2001; Jaiswal et al. 2011; Jaiswal et al. 2013; Müller et al. 2020; supported by Bagci et al. 2020)
ARHGEF15 (Fukushima et al. 2016; Müller et al. 2020)
ARHGEF17 (Rümenapp et al. 2002; Mitin et al. 2012; Müller et al. 2020; supported by Bagci et al. 2020)
ARHGEF19 (Wang et al. 2004; Müller et al. 2020)
ARHGEF25 (Sloan et al. 2012; Müller et al. 2020)
ARHGEF28 (van Horck et al. 2001; Jaiswal et al. 2011; Jaiswal et al. 2013; Müller et al. 2020)
ECT2 (Tatsumoto et al. 1999; Müller et al. 2020; supported by Bagci et al. 2020)
MCF2 (Sloan et al. 2012; Jaiswal et al. 2013; Müller et al. 2020)
NET1 (Alberts and Treisman 1998; Müller et al. 2020)
NGEF (Zhang et al. 2007; Müller et al. 2020; supported by Bagci et al. 2020)
OBSCN (Ford Speelman et al. 2009)
PLEKHG5 (de Toledo et al. 2001; Liu and Horowitz 2006; Müller et al. 2020)
PLEKHG6 (Müller et al. 2020)
RASGRF2 (Müller et al. 2020)
TRIO (TRIO and KALRN possess two GEF domains, the N-terminal GEF1 and the C-terminal GEF2; Debant et al. 1996 demonstrated activation of RHOA by the C-terminal GEF2 domain of TRIO, while Peurois et al. 2017 demonstrated activation of RHOA by the N-terminal GEF1 domain of TRIO in the presence of membranes, Williams et al. 2007 showed that in C-elegans GNAQ-mediated activation of TRIO results in RHOA activation that drived locomotion and egg laying, and Bagci et al. 2020 reported binding of inactive RHOA mutant to the full-length TRIO; Debant et al. 1996 and Jaiswal et al. 2013 showed that the N-terminal GEF1 domain of TRIO does not activate RHOA; Müller et al. 2020 detected no activation of RHOA in the presence of full-length TRIO, but in this study HEK293 cells were used, which were previously shown by Williams et al. 2007 to have a low TRIO-activation ability downstream of GNAQ)
VAV1 (Heo et al. 2005; Müller et al. 2020)
VAV2 (Heo et al. 2005; Sloan et al. 2012; Schuebel et al. 1998; Jaiswal et al. 2013; Müller et al. 2020; supported by Bagci et al. 2020)
VAV3 (Movilla and Bustelo 1999, Sachdev et al. 2002; Müller et al. 2020)
They are annotated as RHOA GEF set members.
The following GEFs were shown to bind and activate RHOA in some but not all studies, or have not been sufficiently studied, and are annotated as candidate RHOA GEFs:
ABR (Chuang et al. 1995, Manser et al. 1998: activation of RHOA; Müller et al. 2020: no activation of RHOA)
ARHGEF4 (Müller et al. 2020: activation of RHOA; Gotthardt and Ahmadian 2007, Anderson and Hamann 2012, Jaiswal et al. 2013: no activation of RHOA)
ARHGEF7 (Manser et al. 1998: activation of RHOA; Jones and Katan 2007, Müller et al. 2020: no activation of RHOA; Bagci et al. 2020: no binding to RHOA)
ARHGEF10L (Winkler et al. 2005: activation of RHOA; Müller et al. 2020: no activation of RHOA)
ARHGEF18 (Niu et al. 2003, Blomquist et al. 2000: activation of RHOA; Müller et al. 2020: no activation of RHOA)
ARHGEF40 (Curtis et al. 2004: activation of RHOA; Müller et al. 2020: no activation of RHOA; Bagci et al. 2020: no binding to RHOA)
BCR (Chuang et al. 1995: activation of RHOA; Bagci et al. 2020: binding to RHOA; Müller et al. 2020: no activation of RHOA)
DEF6 (Mavrakis et al. 2004: probable GEF, in vitro GEF activity not tested)
DOCK2 (Toffali et al. 2017: inconclusive; Müller et al. 2020: no activation of RHOA)
FARP1 (Koyano et al. 2001: activation of RHOA; Müller et al. 2020: no activation of RHOA; Bagci et al. 2020: inconclusive binding to RHOA)
KALRN (Penzes et al. 2001: the C-terminal GEF2 domain of KALRN binds RHOA and activates it, although activation was not demonstrated in vitro, while the N-terminal GEF1 domain of KALRN does not bind to RHOA; Wu et al. 2013: no effect on RHOA activity in mice with heterozygous Kalrn gene knockout; Müller et al. 2020: no activation of RHOA by full-length KALRN)
MCF2L (Whitehead et al. 1999, Sloan et al. 2012, Jaiswal et al. 2013, Müller et al. 2020: activation of RHOA; Bagci et al. 2020: no binding to RHOA)
PLEKHG3 (Bagci et al. 2020: binding to RHOA, RHOA activation not tested; Müller et al. 2020: no activation of RHOA)
PLEKHG4 (Gupta et al. 2013: activation of RHOA; Müller et al. 2020: no activation of RHOA; Bagci et al. 2020: no binding to RHOA)
PREX1 (Jaiswal et al. 2013: activation of RHOA; Marei et al. 2016, Müller et al. 2020: no activation of RHOA)
PREX2 (Müller et al. 2020: activation of RHOA; Joseph and Norris 2005: no activation of RHOA)
TIAM1 (Michiels et al. 1995: activation of RHOA; Jaiswal et al. 2013, Müller et al. 2020: no activation of RHOA; Bagci et al. 2020: no binding to RHOA)
The following GEFs do not act on RHOA or have been reported to not bind to RHOA, as indicated, without testing for activity:
ALS2 (Müller et al. 2020)
ARHGEF6 (Ramakers et al. 2012; Müller et al. 2020)
ARHGEF9 (Reid et al. 1999; Jaiswal et al. 2013; Müller et al. 2020)
ARHGEF16 (Hiramoto Yamaki et al. 2010; Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
ARHGEF26 (Ellerbroek et al. 2004; Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
ARHGEF39 (Müller et al. 2020)
DNMBP (Jaiswal et al. 2013; Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
DOCK1 (Côté and Vuori 2002; Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
DOCK3 (Müller et al. 2020)
DOCK4 (Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
DOCK5 (Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
DOCK6 (Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
DOCK7 (Müller et al. 2020; no binding to RHOA byBagci et al. 2020)
DOCK8 (Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
DOCK9 (Ruiz Lafuente et al. 2015; Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
DOCK10 (Ruiz Lafuente et al. 2015; Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
DOCK11 (Ruiz Lafuente et al. 2015; Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
ECT2L (Müller et al. 2020)
FARP2 (Kubo et al. 2002; Müller et al. 2020);
FGD1 (Olson et al. 1996; Müller et al. 2020)
FGD2 (Huber et al. 2008; Müller et al. 2020)
FGD3 (Müller et al. 2020)
FGD5 (Müller et al. 2020)
FGD6 (Müller et al. 2020)
FGD4 (Umikawa et al. 1999; Müller et al. 2020)
GNA13 (Teo et al. 2016)
ITSN1 (Hussain et al. 2001; Jaiswal et al. 2013; Müller et al. 2020)
ITSN2 (Müller et al. 2020)
MCF2L2 (Müller et al. 2020)
PLEKHG1 (Abiko et al. 2015; Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
PLEKHG2 (Ueda et al. 2008; Müller et al. 2020; no binding to RHOA by Bagci et al. 2020)
PLEKHG4B (Müller et al. 2020)
SOS1 (Nimnual et al. 1998; Müller et al. 2020)
SOS2 (Nimnual et al. 1998; Müller et al. 2020)
SPATA13 (Hamann et al. 2007; Kawasaki et al. 2007; Bristow et al. 2009; Müller et al. 2020)
SWAP70 (Ocana Morgner et al. 2009; no binding to RHOA by Bagci et al. 2020)
TIAM2 (Müller et al. 2020)
Identifier: R-HSA-9013159
Species:
Homo sapiens
Compartment:
plasma membrane,
cytosol
The following guanine nucleotide exchange factors (GEFs) were shown to bind CDC42 and catalyze GDP to GTP exchange on CDC42, resulting in formation of the active CDC42:GTP complex (the high throughput study by Bagci et al. 2020 examined binding of GEFs to inactive CDC42 mutants without testing for CDC42-directed GEF activity and is cited as supporting evidence):
ARHGEF4 (also known as Asef) (Itoh et al. 2008; Anderson and Hamann 2012; Gotthardt and Ahmadian 2007; Jaiswal et al. 2013; Müller et al. 2020)
ARHGEF9 (also known as hPEM2) (Reid et al. 1999; Jaiswal et al. 2013; Müller et al. 2020)
ARHGEF15 (Fukushima et al. 2016; Müller et al. 2020)
DEF6 (Mavrakis et al. 2004)
DNMBP (also known as Tuba) (Salazar et al. 2003; Jaiswal et al. 2013; Müller et al. 2020; supported by Bagci et al. 2020)
FGD1 (Olson et al. 1996; Müller et al. 2020)
FGD2 (Huber et al. 2008; Müller et al. 2020)
FGD3 (Hayakawa et al. 2008; Müller et al. 2020)
FGD4 (Umikawa et al. 1999; Müller et al. 2020)
MCF2 (Ueda et al. 2004; Jaiswal et al. 2013; Müller et al. 2020)
MCF2L (Ueda et al. 2004; Whitehead et al. 1999; Jaiswal et al. 2013; Müller et al. 2020; supported by Bagci et al. 2020)
PLEKHG1 (Abiko et al. 2015; Müller et al. 2020; supported by Bagci et al. 2020)
PLEKHG3 (Nguyen et al. 2016; Müller et al. 2020; supported by Bagci et al. 2020)
PLEKHG4 (Gupta et al. 2013; Müller et al. 2020; supported by Bagci et al. 2020)
PLEKHG4B (Müller et al. 2020)
SPATA13 (Hamann et al. 2007; Kawasaki et al. 2007; Bristow et al. 2009; Müller et al. 2020)
The following GEFs; annotated as CDC42 candidate GEFs; were shown to activate CDC42 by some but not all studies (the high throughput study by Bagci et al. 2020 examined binding of GEFs to inactive CDC42 mutants without testing for CDC42-directed GEF activity and is cited as supporting evidence):
ABR (Chuang et al. 1995: CDC42-directed GEF activity; Müller et al. 2020: no CDC42-directed GEF activity)
ARHGEF5 (Xie et al. 2005: CDC42 directed GEF activity; Müller et al. 2020: no CDC42 directed GEF activity; Bagci et al. 2020: no binding to inactive CDC42)
ARHGEF6 (also known as alphaPIX or KIAA006) (Manser et al. 1998, Ramakers et al. 2012, Meseke et al. 2013: CDC42-directed GEF activity; Müller et al. 2020: no CDC42 directed GEF activity)
ARHGEF10 (Müller et al. 2020: CDC42 directed GEF activity; Mohl et al. 2006: no CDC42-directed GEF activity)
ARHGEF11 (Bagci et al. 2020: binding to inactive CDC42; Rümenapp et al. 1999, Jaiswal et al. 2011, Jaiswal et al. 2013, Müller et al. 2020: no CDC42 directed GEF activity)
ARHGEF12 (Bagci et al. 2020: binding to inactive CDC42; Reuther et al. 2001, Jaiswal et al. 2011, Jaiswal et al. 2013, Müller et al. 2020: no CDC42 directed GEF activity)
ARHGEF16 (Bagci et al. 2020: binding to inactive CDC42; Hiramoto Yamaki et al. 2010, Müller et al. 2020: no CDC42 directed GEF activity)
ARHGEF19 (Wang et al. 2004: CDC42-directed GEF activity; Müller et al. 2020: no CDC42-directed GEF activity)
ARHGEF25 (Guo et al. 2003: CDC42-directed GEF activity; Müller et al. 2020: no CDC42-directed GEF activity)
ARHGEF26 (Müller et al. 2020: CDC42 directed GEF activity; Bagci et al. 2020: binding to inactive CDC42; Ellerbroek et al. 2004: no CDC42 directed GEF activity)
BCR (Chuang et al. 1995; Korus et al. 2002: CDC42 directed GEF activity; Müller et al. 2020: no CDC42 directed GEF activity; Bagci et al. 2020: no binding to inactive CDC42)
DOCK6 (Miyamoto et al. 2007: CDC42 directed GEF activity; Müller et al. 2020: no CDC42 directed GEF activity; Bagci et al. 2020: no binding to inactive CDC42)
DOCK7 (Kukimoto Niino et al. 2019, Wilkes et al. 2014, Zhou et al. 2013, Yamauchi et al. 2008: CDC42-directed GEF activity; Müller et al. 2020: no CDC42-directed GEF activity; Bagci et al. 2020: no binding to inactive CDC42)
DOCK8 (Ruusala and Aspenstrom 2004, Harada et al. 2012, Martins et al. 2016, Xu et al. 2017: CDC42-directed GEF activity; Bagci et al. 2020: binding to inactive CDC42; Müller et al. 2020: no CDC42-directed GEF activity)
DOCK9 (also known as Zizimin1) (Meller et al. 2002, Cote and Vuori 2002, Kulkarni et al. 2011: CDC42-directed GEF activity; Bagci et al. 2020: binding to inactive CDC42; Müller et al. 2020: no CDC42-directed GEF activity)
DOCK10 (Gadea et al. 2008, Ruiz Lafuente et al. 2015: CDC42-directed GEF activity; Bagci et al. 2020: binding to inactive CDC42; Müller et al. 2020: no CDC42-directed GEF activity)
DOCK11 (Lin et al. 2006: CDC42-directed GEF activity; Bagci et al. 2020: binding to inactive CDC42; Müller et al. 2020: no CDC42-directed GEF activity)
ECT2 (Tatsumoto et al. 1999, Fortin et al. 2012: CDC42 directed GEF activity; Müller et al. 2020: no CDC42 directed GEF activity; Bagci et al. 2020: no binding to inactive CDC42)
FARP1 (Amado Azevedo et al. 2017: CDC42 directed GEF activity; Müller et al. 2020: no CDC42 directed GEF activity; Bagci et al. 2020: no binding to inactive CDC42)
GNA13 (Yan et al. 2015: GNA13 increases the amount of GTP-bound CDC42, but the evidence for direct effect is lacking)
ITSN1 (Hussain et al. 2001, Jaiswal et al. 2013: CDC42-directed GEF activity; Müller et al. 2020: no CDC42-directed GEF activity)
NGEF (Zhang et al. 2007: CDC42 directed GEF activity; Müller et al. 2020: no CDC42 directed GEF activity; Bagci et al. 2020: no binding to inactive CDC42)
PLEKHG2 (Ueda et al. 2008: CDC42-directed GEF activity; Bagci et al. 2020: binding to inactive CDC42; Müller et al. 2020: no CDC42-directed GEF activity)
PREX1 (Jaiswal et al. 2013: CDC42 directed GEF activity; Marei et al. 2016, Müller et al. 2020: no CDC42 directed GEF activity)
PREX2 (Müller et al. 2020: CDC42-directed GEF activity; Joseph and Norris 2005: no CDC42-directed GEF activity)
RASGRF2 (Müller et al. 2020: CDC42-directed GEF activity; Calvo et al. 2011: no CDC42-directed GEF activity)
TIAM1 (Michiels et al. 1995: CDC42 directed GEF activity; Itoh et al. 2008, Jaiswal et al. 2013, Müller et al. 2020: no CDC42 directed GEF activity; Bagci et al. 2020: no binding to inactive CDC42)
TRIO (Peurois et al. 2017, Fortin et al. 2012, Jaiswal et al. 2013: CDC42 directed GEF activity, the presence of membrane may be necessary; Debant et al. 1996: no CDC42-directed GEF activity of either the N-terminal GEF1 domain or the C-terminal GEF2 domain of TRIO in vitro; Müller et al. 2020: no CDC42 directed GEF activity of the full-length TRIO; Bagci et al. 2020: no binding of full-length TRIO to inactive CDC42)
VAV2 (Itoh et al. 2008, Aoki et al. 2005, Heo et al. 2005, Jaiswal et al. 2013: CDC42-directed GEF activity; Bagci et al. 2020: binding to inactive CDC42; Müller et al. 2020: no CDC42 directed GEF activity)
VAV3 (Sachdev et al. 2002; Aoki et al. 2005: CDC42 directed GEF activity; Movilla and Bustelo 1999, Müller et al. 2020: no CDC42 directed GEF activity)
The following GEFs do not act on CDC42 or were shown to not bind to inactive CDC42 mutant in the high throughput screen by Bagci et al. 2020):
AKAP13 (Zheng et al. 1995; Müller et al. 2020; Bagci et al. 2020: no binding to inactive CDC42)
ALS2 (Müller et al. 2020)
ARHGEF1 (Hart et al. 1996; Jaiswal et al. 2013; Jaiswal et al. 2011; Müller et al. 2020; Bagci et al. 2020: no binding to inactive CDC42)
ARHGEF2 (Krendel et al. 2002; Müller et al. 2020; Bagci et al. 2020: no binding to inactive CDC42)
ARHGEF3 (Arthur et al. 2002; Müller et al. 2020)
ARHGEF7 (Manser et al. 1998; Müller et al. 2020; Bagci et al. 2020: no binding to inactive CDC42)
ARHGEF10L (Winkler et al. 2005; Müller et al. 2020)
ARHGEF17 (Rümenapp et al. 2002; Müller et al. 2020; Bagci et al. 2020: no binding to inactive CDC42)
ARHGEF18 (Niu et al. 2003; Blomquist et al. 2000; Müller et al. 2020)
ARHGEF28 (van Horck et al. 2001; Jaiswal et al. 2011; Jaiswal et al. 2013; Müller et al. 2020)
ARHGEF39 (Müller et al. 2020)
ARHGEF40 (Curtis et al. 2004; Müller et al. 2020; Bagci et al. 2020: no binding to inactive CDC42)
DOCK1 (Cote and Vuori 2002; Müller et al. 2020; Bagci et al. 2020: no binding to inactive CDC42)
DOCK2 (Kulkarni et al. 2011; Kwofie and Skowronski 2008; Müller et al. 2020)
DOCK3 (Kwofie and Skowronski 2008; Müller et al. 2020)
DOCK4 (Kwofie and Skowronski 2008; Abraham et al. 2015; Müller et al. 2020; Bagci et al. 2020: no binding to inactive CDC42)
DOCK5 (Vives et al. 2011; Müller et al. 2020; Bagci et al. 2020: no binding to inactive CDC42)
ECT2L (Müller et al. 2020)
FARP2 (Kubo et al. 2002; Müller et al. 2020)
FGD5 (Müller et al. 2020)
FGD6 (Müller et al. 2020)
ITSN2 (Müller et al. 2020)
KALRN (Penzes et al. 2001; Müller et al. 2020)
MCF2L2 (Müller et al. 2020)
NET1 (Alberts and Treisman 1998; Müller et al. 2020)
OBSCN (Ford Speelman et al. 2009)
PLEKHG5 (De Toledo et al. 2001; Müller et al. 2020)
PLEKHG6 (Müller et al. 2020)
PLEKHG7 (Müller et al. 2020)
RASGRF1 (Müller et al. 2020)
SOS1 (Nimnual et al. 1998; Müller et al. 2020)
SOS2 (Nimnual et al. 1998; Müller et al. 2020)
SWAP70 (Bagci et al. 2020: no binding to inactive CDC42)
TIAM2 (Müller et al. 2020)
VAV1 (Aghazadeh et al. 2000; Müller et al. 2020)
ARHGEF4 (also known as Asef) (Itoh et al. 2008; Anderson and Hamann 2012; Gotthardt and Ahmadian 2007; Jaiswal et al. 2013; Müller et al. 2020)
ARHGEF9 (also known as hPEM2) (Reid et al. 1999; Jaiswal et al. 2013; Müller et al. 2020)
ARHGEF15 (Fukushima et al. 2016; Müller et al. 2020)
DEF6 (Mavrakis et al. 2004)
DNMBP (also known as Tuba) (Salazar et al. 2003; Jaiswal et al. 2013; Müller et al. 2020; supported by Bagci et al. 2020)
FGD1 (Olson et al. 1996; Müller et al. 2020)
FGD2 (Huber et al. 2008; Müller et al. 2020)
FGD3 (Hayakawa et al. 2008; Müller et al. 2020)
FGD4 (Umikawa et al. 1999; Müller et al. 2020)
MCF2 (Ueda et al. 2004; Jaiswal et al. 2013; Müller et al. 2020)
MCF2L (Ueda et al. 2004; Whitehead et al. 1999; Jaiswal et al. 2013; Müller et al. 2020; supported by Bagci et al. 2020)
PLEKHG1 (Abiko et al. 2015; Müller et al. 2020; supported by Bagci et al. 2020)
PLEKHG3 (Nguyen et al. 2016; Müller et al. 2020; supported by Bagci et al. 2020)
PLEKHG4 (Gupta et al. 2013; Müller et al. 2020; supported by Bagci et al. 2020)
PLEKHG4B (Müller et al. 2020)
SPATA13 (Hamann et al. 2007; Kawasaki et al. 2007; Bristow et al. 2009; Müller et al. 2020)
The following GEFs; annotated as CDC42 candidate GEFs; were shown to activate CDC42 by some but not all studies (the high throughput study by Bagci et al. 2020 examined binding of GEFs to inactive CDC42 mutants without testing for CDC42-directed GEF activity and is cited as supporting evidence):
ABR (Chuang et al. 1995: CDC42-directed GEF activity; Müller et al. 2020: no CDC42-directed GEF activity)
ARHGEF5 (Xie et al. 2005: CDC42 directed GEF activity; Müller et al. 2020: no CDC42 directed GEF activity; Bagci et al. 2020: no binding to inactive CDC42)
ARHGEF6 (also known as alphaPIX or KIAA006) (Manser et al. 1998, Ramakers et al. 2012, Meseke et al. 2013: CDC42-directed GEF activity; Müller et al. 2020: no CDC42 directed GEF activity)
ARHGEF10 (Müller et al. 2020: CDC42 directed GEF activity; Mohl et al. 2006: no CDC42-directed GEF activity)
ARHGEF11 (Bagci et al. 2020: binding to inactive CDC42; Rümenapp et al. 1999, Jaiswal et al. 2011, Jaiswal et al. 2013, Müller et al. 2020: no CDC42 directed GEF activity)
ARHGEF12 (Bagci et al. 2020: binding to inactive CDC42; Reuther et al. 2001, Jaiswal et al. 2011, Jaiswal et al. 2013, Müller et al. 2020: no CDC42 directed GEF activity)
ARHGEF16 (Bagci et al. 2020: binding to inactive CDC42; Hiramoto Yamaki et al. 2010, Müller et al. 2020: no CDC42 directed GEF activity)
ARHGEF19 (Wang et al. 2004: CDC42-directed GEF activity; Müller et al. 2020: no CDC42-directed GEF activity)
ARHGEF25 (Guo et al. 2003: CDC42-directed GEF activity; Müller et al. 2020: no CDC42-directed GEF activity)
ARHGEF26 (Müller et al. 2020: CDC42 directed GEF activity; Bagci et al. 2020: binding to inactive CDC42; Ellerbroek et al. 2004: no CDC42 directed GEF activity)
BCR (Chuang et al. 1995; Korus et al. 2002: CDC42 directed GEF activity; Müller et al. 2020: no CDC42 directed GEF activity; Bagci et al. 2020: no binding to inactive CDC42)
DOCK6 (Miyamoto et al. 2007: CDC42 directed GEF activity; Müller et al. 2020: no CDC42 directed GEF activity; Bagci et al. 2020: no binding to inactive CDC42)
DOCK7 (Kukimoto Niino et al. 2019, Wilkes et al. 2014, Zhou et al. 2013, Yamauchi et al. 2008: CDC42-directed GEF activity; Müller et al. 2020: no CDC42-directed GEF activity; Bagci et al. 2020: no binding to inactive CDC42)
DOCK8 (Ruusala and Aspenstrom 2004, Harada et al. 2012, Martins et al. 2016, Xu et al. 2017: CDC42-directed GEF activity; Bagci et al. 2020: binding to inactive CDC42; Müller et al. 2020: no CDC42-directed GEF activity)
DOCK9 (also known as Zizimin1) (Meller et al. 2002, Cote and Vuori 2002, Kulkarni et al. 2011: CDC42-directed GEF activity; Bagci et al. 2020: binding to inactive CDC42; Müller et al. 2020: no CDC42-directed GEF activity)
DOCK10 (Gadea et al. 2008, Ruiz Lafuente et al. 2015: CDC42-directed GEF activity; Bagci et al. 2020: binding to inactive CDC42; Müller et al. 2020: no CDC42-directed GEF activity)
DOCK11 (Lin et al. 2006: CDC42-directed GEF activity; Bagci et al. 2020: binding to inactive CDC42; Müller et al. 2020: no CDC42-directed GEF activity)
ECT2 (Tatsumoto et al. 1999, Fortin et al. 2012: CDC42 directed GEF activity; Müller et al. 2020: no CDC42 directed GEF activity; Bagci et al. 2020: no binding to inactive CDC42)
FARP1 (Amado Azevedo et al. 2017: CDC42 directed GEF activity; Müller et al. 2020: no CDC42 directed GEF activity; Bagci et al. 2020: no binding to inactive CDC42)
GNA13 (Yan et al. 2015: GNA13 increases the amount of GTP-bound CDC42, but the evidence for direct effect is lacking)
ITSN1 (Hussain et al. 2001, Jaiswal et al. 2013: CDC42-directed GEF activity; Müller et al. 2020: no CDC42-directed GEF activity)
NGEF (Zhang et al. 2007: CDC42 directed GEF activity; Müller et al. 2020: no CDC42 directed GEF activity; Bagci et al. 2020: no binding to inactive CDC42)
PLEKHG2 (Ueda et al. 2008: CDC42-directed GEF activity; Bagci et al. 2020: binding to inactive CDC42; Müller et al. 2020: no CDC42-directed GEF activity)
PREX1 (Jaiswal et al. 2013: CDC42 directed GEF activity; Marei et al. 2016, Müller et al. 2020: no CDC42 directed GEF activity)
PREX2 (Müller et al. 2020: CDC42-directed GEF activity; Joseph and Norris 2005: no CDC42-directed GEF activity)
RASGRF2 (Müller et al. 2020: CDC42-directed GEF activity; Calvo et al. 2011: no CDC42-directed GEF activity)
TIAM1 (Michiels et al. 1995: CDC42 directed GEF activity; Itoh et al. 2008, Jaiswal et al. 2013, Müller et al. 2020: no CDC42 directed GEF activity; Bagci et al. 2020: no binding to inactive CDC42)
TRIO (Peurois et al. 2017, Fortin et al. 2012, Jaiswal et al. 2013: CDC42 directed GEF activity, the presence of membrane may be necessary; Debant et al. 1996: no CDC42-directed GEF activity of either the N-terminal GEF1 domain or the C-terminal GEF2 domain of TRIO in vitro; Müller et al. 2020: no CDC42 directed GEF activity of the full-length TRIO; Bagci et al. 2020: no binding of full-length TRIO to inactive CDC42)
VAV2 (Itoh et al. 2008, Aoki et al. 2005, Heo et al. 2005, Jaiswal et al. 2013: CDC42-directed GEF activity; Bagci et al. 2020: binding to inactive CDC42; Müller et al. 2020: no CDC42 directed GEF activity)
VAV3 (Sachdev et al. 2002; Aoki et al. 2005: CDC42 directed GEF activity; Movilla and Bustelo 1999, Müller et al. 2020: no CDC42 directed GEF activity)
The following GEFs do not act on CDC42 or were shown to not bind to inactive CDC42 mutant in the high throughput screen by Bagci et al. 2020):
AKAP13 (Zheng et al. 1995; Müller et al. 2020; Bagci et al. 2020: no binding to inactive CDC42)
ALS2 (Müller et al. 2020)
ARHGEF1 (Hart et al. 1996; Jaiswal et al. 2013; Jaiswal et al. 2011; Müller et al. 2020; Bagci et al. 2020: no binding to inactive CDC42)
ARHGEF2 (Krendel et al. 2002; Müller et al. 2020; Bagci et al. 2020: no binding to inactive CDC42)
ARHGEF3 (Arthur et al. 2002; Müller et al. 2020)
ARHGEF7 (Manser et al. 1998; Müller et al. 2020; Bagci et al. 2020: no binding to inactive CDC42)
ARHGEF10L (Winkler et al. 2005; Müller et al. 2020)
ARHGEF17 (Rümenapp et al. 2002; Müller et al. 2020; Bagci et al. 2020: no binding to inactive CDC42)
ARHGEF18 (Niu et al. 2003; Blomquist et al. 2000; Müller et al. 2020)
ARHGEF28 (van Horck et al. 2001; Jaiswal et al. 2011; Jaiswal et al. 2013; Müller et al. 2020)
ARHGEF39 (Müller et al. 2020)
ARHGEF40 (Curtis et al. 2004; Müller et al. 2020; Bagci et al. 2020: no binding to inactive CDC42)
DOCK1 (Cote and Vuori 2002; Müller et al. 2020; Bagci et al. 2020: no binding to inactive CDC42)
DOCK2 (Kulkarni et al. 2011; Kwofie and Skowronski 2008; Müller et al. 2020)
DOCK3 (Kwofie and Skowronski 2008; Müller et al. 2020)
DOCK4 (Kwofie and Skowronski 2008; Abraham et al. 2015; Müller et al. 2020; Bagci et al. 2020: no binding to inactive CDC42)
DOCK5 (Vives et al. 2011; Müller et al. 2020; Bagci et al. 2020: no binding to inactive CDC42)
ECT2L (Müller et al. 2020)
FARP2 (Kubo et al. 2002; Müller et al. 2020)
FGD5 (Müller et al. 2020)
FGD6 (Müller et al. 2020)
ITSN2 (Müller et al. 2020)
KALRN (Penzes et al. 2001; Müller et al. 2020)
MCF2L2 (Müller et al. 2020)
NET1 (Alberts and Treisman 1998; Müller et al. 2020)
OBSCN (Ford Speelman et al. 2009)
PLEKHG5 (De Toledo et al. 2001; Müller et al. 2020)
PLEKHG6 (Müller et al. 2020)
PLEKHG7 (Müller et al. 2020)
RASGRF1 (Müller et al. 2020)
SOS1 (Nimnual et al. 1998; Müller et al. 2020)
SOS2 (Nimnual et al. 1998; Müller et al. 2020)
SWAP70 (Bagci et al. 2020: no binding to inactive CDC42)
TIAM2 (Müller et al. 2020)
VAV1 (Aghazadeh et al. 2000; Müller et al. 2020)
Identifier: R-HSA-9013143
Species:
Homo sapiens
Compartment:
plasma membrane,
cytosol
The following guanine nucleotide exchange factors (GEFs) were shown to bind RAC1 and catalyze GDP to GTP exchange on RAC1, resulting in formation of the active RAC1:GTP complex (the high throughput study by Bagci et al. examined binding of GEFs to inactive RAC1 mutant without testing for RAC1-directed GEF activity and is cited as supporting evidence):
DOCK3 (Namekata et al. 2004, Yang et al. 2012, Namekata et al. 2012; Müller et al. 2020)
FGD5 (Müller et al. 2020)
GNA13 (Radhika et al. 2004)
PLEKHG6 (Müller et al. 2020)
PREX1 (Yoshizawa et al. 2005; Dong et al. 2005; Marei et al. 2016; Jaiswal et al. 2013; Müller et al. 2020)
PREX2 (Joseph and Norris 2005; Müller et al. 2020)
RASGRF2 (Schwechter et al. 2013; Müller et al. 2020)
SWAP70 (Shinohara et al. 2002; Gupta et al. 2003; Baranov et al. 2016; Bagci et al. 2020: binding to inactive RAC1)
TIAM2 (Matsuo et al. 2002; Müller et al. 2020)
TRIO (Debant et al. 1996; Moshfegh et al. 2014; Peurois et al. 2017; Jaiswal et al. 2013: RAC1-directed GEF acivity of the N-terminal GEF1 domain of TRIO; Müller et al. 2020: RAC1-directed GEF activity of the full-length TRIO; Bagci et al. 2020: binding of full-length TRIO to inactive RAC1)
VAV1 (Teramoto et al. 1997; Heo et al. 2005; Aghazadeh et al. 2000; Crespo et al. 1997; Müller et al. 2020)
VAV2 (Itoh et al. 2008; Aoki et al. 2005; Heo et al. 2005; Jaiswal et al. 2013; Müller et al. 2020; Bagci et al. 2020: binding to inactive RAC1)
The following GEFs were shown to bind and activate RAC1 in some but not all studies and are annotated as candidate RAC1 GEFs (or were shown, in the high throughput screen by Bagci et al. 2020, to bind to nucleotide free RAC1 mutant, as indicated):
ABR (Chuang et al. 1995: RAC1-directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
ALS2 (Topp et al. 2004, Kanekura et al. 2005, Topp et al. 2005: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF4 (Itoh et al. 2008: RAC1 directed GEF activity; Anderson and Hamann 2012, Gotthardt and Ahmadian 2007, Jaiswal et al. 2013, Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF5 (Xie et al. 2005: RAC1 directed GEF activity; Wang et al. 2009, Müller et al. 2020: no RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF6 (Manser et al. 1998, Ramakers et al. 2012: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF7 (Ten Klooster et al. 2006, Manser et al. 1998: RAC1 directed GEF activity; Bagci et al. 2020: binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF10 (Müller et al. 2020: RAC1 directed GEF activity; Mohl et al. 2006: no RAC1 directed GEF activity)
ARHGEF11 (Bagci et al. 2020: binding to inactive RAC1; Rümenapp et al. 1999; Jaiswal et al. 2011; Jaiswal et al. 2013; Müller et al. 2020: no RAC1 directed GEF activity:)
ARHGEF15 (Fukushima et al. 2016: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF18 (Niu et al. 2003: RAC1 directed GEF activity; Blomquist et al. 2000, Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF19 (Wang et al. 2004: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF25 (Guo et al. 2003: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF39 (Zhou et al. 2018: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
BCR (Chuang et al. 1995: RAC1 directed GEF activity; Bagci et al. 2020 binding to inactive RAC1; Korus et al. 2002, Müller et al. 2020: no RAC1 directed GEF activity)
DEF6 (Mavrakis et al. 2004: activation of RAC1, GEF activity of DEF6 has not been examined in vitro)
DOCK1 (Cote and Vuori 2002, Li et al. 2003: RAC1 directed GEF activity; Bagci et al. 2020: binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
DOCK2 (Kulkarni et al. 2011: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
DOCK4 (Abraham et al. 2015: RAC1-directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
DOCK5 (Omi et al. 2008, Vives et al. 2011, Ferrandez et al. 2017: RAC1 directed GEF activity; Bagci et al. 2020: binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
DOCK6 (Miyamoto et al. 2007: RAC1 directed GEF activity; Bagci et al. 2020: binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
DOCK7 (Kukimoto Niino et al. 2019, Yamauchi et al. 2008, Majewski et al. 2012, Zhou et al. 2013: RAC1 directed GEF activity; Bagci et al. 2020: binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
DOCK8 (Wang et al. 2015: RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
DOCK9 (Bagci et al. 2020: binding to inactive RAC1; Kulkarni et al. 2011, Müller et al. 2020: no RAC1 directed GEF activity)
DOCK10 (Ruiz Lafuente et al. 2015, Müller et al. 2020: RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
DOCK11 (Bagci et al. 2020: binding to inactive RAC1; Lin et al. 2006, Müller et al. 2020: no RAC1 directed GEF activity)
ECT2 (Tatsumoto et al. 1999: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
FARP1 (Cheadle and Biederer 2012: RAC1-directed GEF activity; Bagci et al. 2020: binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
FARP2 (Kubo et al. 2002: RAC1-directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
KALRN (Penzes et al. 2003, Wu et al. 2013: RAC1-directed GEF activity when using the N-terminal GEF1 domain of KALRN; Müller et al. 2020: no RAC1 directed GEF activity when using full-length KALRN)
MCF2 (Jaiswal et al. 2013, Müller et al. 2020: RAC1 directed GEF activity; Reuther et al. 2001: no RAC1 directed GEF activity);
MCF2L (Bagci et al. 2020: binding to inactive RAC1; Whitehead et al. 1999; Jaiswal et al. 2013; Müller et al. 2020: no RAC1 directed GEF activity)
NGEF (Zhang et al. 2007: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
PLEKHG1 (Abiko et al. 2015: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
PLEKHG2 (Ueda et al. 2008: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
PLEKHG3 (Nguyen et al. 2016: RAC1-directed GEF activity; Bagci et al. 2020: binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
PLEKHG4 (Gupta et al. 2013: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
SOS1 (Nimnual et al. 1998: RAC1 directed GEF activity; Itoh et al. 2008, Müller et al. 2020: no RAC1 directed GEF activity)
SOS2 (Nimnual et al. 1998: RAC1 directed GEF activity; Itoh et al. 2008, Müller et al. 2020: no RAC1 directed GEF activity)
SPATA13 (Kawasaki et al. 2007, Bristow et al. 2009: RAC1 directed GEF activity; Hamann et al. 2007, Müller et al. 2020: no RAC1 directed GEF activity)
TIAM1 (Itoh et al. 2008, Michiels et al. 1995, Haeusler et al. 2003, Jaiswal et al. 2013, Müller et al. 2020: RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
VAV3 (Movilla and Bustello 1999, Sachdev et al. 2002, Aoki et al. 2005: RAC1-directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
The following GEFs do not act on RAC1 or were shown to not bind to nucleotide-free RAC1 in the study by Bagci et al. 2020, as indicated:
AKAP13 (Zheng et al. 1995; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF1 (Hart et al. 1996; Jaiswal et al. 2013; Jaiswal et al. 2011; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF2 (Krendel et al. 2002; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF3 (Arthur et al. 2002; Müller et al. 2020)
ARHGEF9 (Reid et al. 1999; Jaiswal et al. 2013; Müller et al. 2020)
ARHGEF10L (Winkler et al. 2005; Müller et al. 2020)
ARHGEF12 (Reuther et al. 2001; Jaiswal et al. 2013; Jaiswal et al. 2011; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF16 (Hiramoto Yamaki et al. 2010; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF17 (Rümenapp et al. 2002; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF26 (Ellerbroek et al. 2004; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF28 (van Horck et al. 2001; Jaiswal et al. 2013; Jaiswal et al. 2011; Müller et al. 2020)
ARHGEF40 (Curtis et al. 2004; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
DNMBP (Jaiswal et al. 2013; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ECT2L (Müller et al. 2020)
FGD1 (Olson et al. 1996; Müller et al. 2020)
FGD2 (Huber et al. 2008; Müller et al. 2020)
FGD3 (Müller et al. 2020)
FGD4 (Umikawa et al. 1999; Müller et al. 2020)
FGD6 (Müller et al. 2020)
ITSN1 (Hussain et al. 2001; Jaiswal et al. 2013; Müller et al. 2020)
ITSN2 (Müller et al. 2020)
MCF2L2 (Müller et al. 2020)
NET1 (Alberts and Treisman 1998; Müller et al. 2020)
OBSCN (Ford Speelman et al. 2009)
PLEKHG4B (Müller et al. 2020)
PLEKHG5 (De Toledo et al. 2004; Müller et al. 2020)
PLEKHG7 (Müller et al. 2020)
RASGRF1 (Müller et al. 2020)
DOCK3 (Namekata et al. 2004, Yang et al. 2012, Namekata et al. 2012; Müller et al. 2020)
FGD5 (Müller et al. 2020)
GNA13 (Radhika et al. 2004)
PLEKHG6 (Müller et al. 2020)
PREX1 (Yoshizawa et al. 2005; Dong et al. 2005; Marei et al. 2016; Jaiswal et al. 2013; Müller et al. 2020)
PREX2 (Joseph and Norris 2005; Müller et al. 2020)
RASGRF2 (Schwechter et al. 2013; Müller et al. 2020)
SWAP70 (Shinohara et al. 2002; Gupta et al. 2003; Baranov et al. 2016; Bagci et al. 2020: binding to inactive RAC1)
TIAM2 (Matsuo et al. 2002; Müller et al. 2020)
TRIO (Debant et al. 1996; Moshfegh et al. 2014; Peurois et al. 2017; Jaiswal et al. 2013: RAC1-directed GEF acivity of the N-terminal GEF1 domain of TRIO; Müller et al. 2020: RAC1-directed GEF activity of the full-length TRIO; Bagci et al. 2020: binding of full-length TRIO to inactive RAC1)
VAV1 (Teramoto et al. 1997; Heo et al. 2005; Aghazadeh et al. 2000; Crespo et al. 1997; Müller et al. 2020)
VAV2 (Itoh et al. 2008; Aoki et al. 2005; Heo et al. 2005; Jaiswal et al. 2013; Müller et al. 2020; Bagci et al. 2020: binding to inactive RAC1)
The following GEFs were shown to bind and activate RAC1 in some but not all studies and are annotated as candidate RAC1 GEFs (or were shown, in the high throughput screen by Bagci et al. 2020, to bind to nucleotide free RAC1 mutant, as indicated):
ABR (Chuang et al. 1995: RAC1-directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
ALS2 (Topp et al. 2004, Kanekura et al. 2005, Topp et al. 2005: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF4 (Itoh et al. 2008: RAC1 directed GEF activity; Anderson and Hamann 2012, Gotthardt and Ahmadian 2007, Jaiswal et al. 2013, Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF5 (Xie et al. 2005: RAC1 directed GEF activity; Wang et al. 2009, Müller et al. 2020: no RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF6 (Manser et al. 1998, Ramakers et al. 2012: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF7 (Ten Klooster et al. 2006, Manser et al. 1998: RAC1 directed GEF activity; Bagci et al. 2020: binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF10 (Müller et al. 2020: RAC1 directed GEF activity; Mohl et al. 2006: no RAC1 directed GEF activity)
ARHGEF11 (Bagci et al. 2020: binding to inactive RAC1; Rümenapp et al. 1999; Jaiswal et al. 2011; Jaiswal et al. 2013; Müller et al. 2020: no RAC1 directed GEF activity:)
ARHGEF15 (Fukushima et al. 2016: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF18 (Niu et al. 2003: RAC1 directed GEF activity; Blomquist et al. 2000, Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF19 (Wang et al. 2004: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF25 (Guo et al. 2003: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
ARHGEF39 (Zhou et al. 2018: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
BCR (Chuang et al. 1995: RAC1 directed GEF activity; Bagci et al. 2020 binding to inactive RAC1; Korus et al. 2002, Müller et al. 2020: no RAC1 directed GEF activity)
DEF6 (Mavrakis et al. 2004: activation of RAC1, GEF activity of DEF6 has not been examined in vitro)
DOCK1 (Cote and Vuori 2002, Li et al. 2003: RAC1 directed GEF activity; Bagci et al. 2020: binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
DOCK2 (Kulkarni et al. 2011: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
DOCK4 (Abraham et al. 2015: RAC1-directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
DOCK5 (Omi et al. 2008, Vives et al. 2011, Ferrandez et al. 2017: RAC1 directed GEF activity; Bagci et al. 2020: binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
DOCK6 (Miyamoto et al. 2007: RAC1 directed GEF activity; Bagci et al. 2020: binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
DOCK7 (Kukimoto Niino et al. 2019, Yamauchi et al. 2008, Majewski et al. 2012, Zhou et al. 2013: RAC1 directed GEF activity; Bagci et al. 2020: binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
DOCK8 (Wang et al. 2015: RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
DOCK9 (Bagci et al. 2020: binding to inactive RAC1; Kulkarni et al. 2011, Müller et al. 2020: no RAC1 directed GEF activity)
DOCK10 (Ruiz Lafuente et al. 2015, Müller et al. 2020: RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
DOCK11 (Bagci et al. 2020: binding to inactive RAC1; Lin et al. 2006, Müller et al. 2020: no RAC1 directed GEF activity)
ECT2 (Tatsumoto et al. 1999: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
FARP1 (Cheadle and Biederer 2012: RAC1-directed GEF activity; Bagci et al. 2020: binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
FARP2 (Kubo et al. 2002: RAC1-directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
KALRN (Penzes et al. 2003, Wu et al. 2013: RAC1-directed GEF activity when using the N-terminal GEF1 domain of KALRN; Müller et al. 2020: no RAC1 directed GEF activity when using full-length KALRN)
MCF2 (Jaiswal et al. 2013, Müller et al. 2020: RAC1 directed GEF activity; Reuther et al. 2001: no RAC1 directed GEF activity);
MCF2L (Bagci et al. 2020: binding to inactive RAC1; Whitehead et al. 1999; Jaiswal et al. 2013; Müller et al. 2020: no RAC1 directed GEF activity)
NGEF (Zhang et al. 2007: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
PLEKHG1 (Abiko et al. 2015: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
PLEKHG2 (Ueda et al. 2008: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
PLEKHG3 (Nguyen et al. 2016: RAC1-directed GEF activity; Bagci et al. 2020: binding to inactive RAC1; Müller et al. 2020: no RAC1 directed GEF activity)
PLEKHG4 (Gupta et al. 2013: RAC1 directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
SOS1 (Nimnual et al. 1998: RAC1 directed GEF activity; Itoh et al. 2008, Müller et al. 2020: no RAC1 directed GEF activity)
SOS2 (Nimnual et al. 1998: RAC1 directed GEF activity; Itoh et al. 2008, Müller et al. 2020: no RAC1 directed GEF activity)
SPATA13 (Kawasaki et al. 2007, Bristow et al. 2009: RAC1 directed GEF activity; Hamann et al. 2007, Müller et al. 2020: no RAC1 directed GEF activity)
TIAM1 (Itoh et al. 2008, Michiels et al. 1995, Haeusler et al. 2003, Jaiswal et al. 2013, Müller et al. 2020: RAC1 directed GEF activity; Bagci et al. 2020: no binding to inactive RAC1)
VAV3 (Movilla and Bustello 1999, Sachdev et al. 2002, Aoki et al. 2005: RAC1-directed GEF activity; Müller et al. 2020: no RAC1 directed GEF activity)
The following GEFs do not act on RAC1 or were shown to not bind to nucleotide-free RAC1 in the study by Bagci et al. 2020, as indicated:
AKAP13 (Zheng et al. 1995; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF1 (Hart et al. 1996; Jaiswal et al. 2013; Jaiswal et al. 2011; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF2 (Krendel et al. 2002; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF3 (Arthur et al. 2002; Müller et al. 2020)
ARHGEF9 (Reid et al. 1999; Jaiswal et al. 2013; Müller et al. 2020)
ARHGEF10L (Winkler et al. 2005; Müller et al. 2020)
ARHGEF12 (Reuther et al. 2001; Jaiswal et al. 2013; Jaiswal et al. 2011; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF16 (Hiramoto Yamaki et al. 2010; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF17 (Rümenapp et al. 2002; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF26 (Ellerbroek et al. 2004; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ARHGEF28 (van Horck et al. 2001; Jaiswal et al. 2013; Jaiswal et al. 2011; Müller et al. 2020)
ARHGEF40 (Curtis et al. 2004; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
DNMBP (Jaiswal et al. 2013; Müller et al. 2020; Bagci et al. 2020: no binding to inactive RAC1)
ECT2L (Müller et al. 2020)
FGD1 (Olson et al. 1996; Müller et al. 2020)
FGD2 (Huber et al. 2008; Müller et al. 2020)
FGD3 (Müller et al. 2020)
FGD4 (Umikawa et al. 1999; Müller et al. 2020)
FGD6 (Müller et al. 2020)
ITSN1 (Hussain et al. 2001; Jaiswal et al. 2013; Müller et al. 2020)
ITSN2 (Müller et al. 2020)
MCF2L2 (Müller et al. 2020)
NET1 (Alberts and Treisman 1998; Müller et al. 2020)
OBSCN (Ford Speelman et al. 2009)
PLEKHG4B (Müller et al. 2020)
PLEKHG5 (De Toledo et al. 2004; Müller et al. 2020)
PLEKHG7 (Müller et al. 2020)
RASGRF1 (Müller et al. 2020)
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