39S subunit binds 28S subunit:mRNA:fMet-tRNA

Stable Identifier
R-BTA-5432421
Type
Reaction [transition]
Species
Bos taurus
Compartment
mitochondrial matrix, mitochondrial inner membrane
ReviewStatus
5/5
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39S subunit binds 28S subunit:mRNA:fMet-tRNA
The 39S ribosomal subunit binds the 28S subunit:mRNA:N-formylmethionyl-tRNA complex, MTIF2 hydrolyzes GTP, then MTIF2, GDP, and MTIF3 dissociate (Liao and Spremulli 1991). (MTIF2 has very low affinity for GDP so it is unclear if MTIF2 and GDP remain associated after hydrolysis of GTP.) The 39S and 28S subunits have 15 intersubunit bridges, only 6 of which are conserved in bacterial ribosomes (Sharma et al. 2003, Greber et al. 2014, Kaushal et al. 2014). The 28S subunit, 39S subunit, and 55S holoribosome associate with the inner mitochondrial membrane independently of translation (Liu and Spremulli 2000).
Literature References
PubMed ID Title Journal Year
24362565 Architecture of the large subunit of the mammalian mitochondrial ribosome

Voigts-Hoffmann, F, Leitner, A, Ban, N, Aebersold, R, Erzberger, JP, Greber, BJ, Bieri, P, Boehringer, D, Leibundgut, M

Nature 2014
14532006 Structure of the mammalian mitochondrial ribosome reveals an expanded functional role for its component proteins

Spremulli, LL, Koc, EC, Agrawal, RK, Sharma, MR, Datta, PP, Booth, TM

Cell 2003
10887179 Interaction of mammalian mitochondrial ribosomes with the inner membrane

Liu, M, Spremulli, L

J. Biol. Chem. 2000
18243113 A single mammalian mitochondrial translation initiation factor functionally replaces two bacterial factors

Spencer, A, Varshney, U, Spremulli, L, Das, G, Gaur, R, Krishna, PD, Grasso, D, Agrawal, RK, Datta, PP

Mol. Cell 2008
24799711 Cryo-EM structure of the small subunit of the mammalian mitochondrial ribosome

Tung, CS, Spremulli, LL, Agrawal, RK, Sanbonmatsu, KY, Sharma, MR, Kaushal, PS, Haque, EM, Booth, TM

Proc. Natl. Acad. Sci. U.S.A. 2014
1939122 Initiation of protein synthesis in animal mitochondria. Purification and characterization of translational initiation factor 2

Spremulli, LL, Liao, HX

J. Biol. Chem. 1991
Participants
Input
Output
Catalyst Activity

GTPase activity of 28S ribosomal subunit:MTIF3:MTIF2:GTP:mRNA:fMet-tRNA(fMet) [mitochondrial inner membrane]

Physical Entity
28S ribosomal subunit:MTIF3:MTIF2:GTP:mRNA:fMet-tRNA(fMet) [mitochondrial inner membrane] (Bos taurus)
Activity
GTPase activity (GO:0003924)
Orthologous Events
39S subunit binds 28S subunit:mRNA:fMet-tRNA (Homo sapiens)
Authored
May, B  (2014-05-09)
Reviewed
Chrzanowska-Lightowlers, ZM  (2014-08-29)
Spremulli, LL  (2014-09-20)
Created
May, B  (2014-05-11)
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