28S subunit binds MITF2:GTP, mRNA, and formylMet-tRNA

Stable Identifier
R-BTA-5432463
Type
Reaction [binding]
Species
Bos taurus
Compartment
mitochondrial matrix, mitochondrial inner membrane
ReviewStatus
5/5
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
28S subunit binds MITF2:GTP, mRNA, and formylMet-tRNA
The 28S subunit in a complex with MTIF3 (IF-3Mt, IF3mt) binds mRNA and, at some point, MTIF2:GTP (IF-2Mt:GTP, IF2mt:GTP) (Liao and Spremulli 1991, Ma and Spremulli 1996, Koc and Spremulli 2002, Christian et al. 2010, Yassin et al. 2011). If an initiation codon is present at the 5' end of the mRNA then N-formylmethionyl-tRNA also binds and a stable, productive initiation complex results (Christian and Spremulli 2010). If no initiation codon is present the 28S subunit slides along the mRNA and then dissociates. MTIF2 in mitochondria appears to perform the functions of both IF1 and IF2 in bacteria (Gaur et al. 2008, Yassin et al. 2011).
Literature References
PubMed ID Title Journal Year
12095986 Identification of mammalian mitochondrial translational initiation factor 3 and examination of its role in initiation complex formation with natural mRNAs

Spremulli, LL, Koc, EC

J. Biol. Chem. 2002
19962967 The effect of spermine on the initiation of mitochondrial protein synthesis

Spremulli, LL, Haque, ME, Christian, BE

Biochem. Biophys. Res. Commun. 2010
20610392 Preferential selection of the 5'-terminal start codon on leaderless mRNAs by mammalian mitochondrial ribosomes

Spremulli, LL, Christian, BE

J. Biol. Chem. 2010
8621449 Expression, purification, and mechanistic studies of bovine mitochondrial translational initiation factor 2

Spremulli, LL, Ma, J

J. Biol. Chem. 1996
18243113 A single mammalian mitochondrial translation initiation factor functionally replaces two bacterial factors

Spencer, A, Varshney, U, Spremulli, L, Das, G, Gaur, R, Krishna, PD, Grasso, D, Agrawal, RK, Datta, PP

Mol. Cell 2008
21368145 Insertion domain within mammalian mitochondrial translation initiation factor 2 serves the role of eubacterial initiation factor 1

Banavali, NK, Spremulli, LL, Haque, ME, Yassin, AS, Agrawal, RK, Datta, PP, Elmore, K

Proc. Natl. Acad. Sci. U.S.A. 2011
1939122 Initiation of protein synthesis in animal mitochondria. Purification and characterization of translational initiation factor 2

Spremulli, LL, Liao, HX

J. Biol. Chem. 1991
Participants
Input
Output
Orthologous Events
28S subunit:MTIF3 binds MTIF2:GTP, mRNA, and formylMet-tRNA (Homo sapiens)
Authored
May, B  (2014-05-09)
Reviewed
Chrzanowska-Lightowlers, ZM  (2014-08-29)
Spremulli, LL  (2014-09-20)
Created
May, B  (2014-05-11)
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