Reactome | Phosphorylated PER binds to CLK

Phosphorylated PER binds to CLK

Stable Identifier

R-DME-538904

Type

Reaction [binding]

Species

Drosophila melanogaster

Compartment

nucleoplasm

ReviewStatus

5/5

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After Timeless (TIM) and Period (PER) reform their heterodimer, PER binds to the E-box bound Clock (CLK) protein. Transcription of the per, tim, vri, and Pdp1 target genes is now deactivated. It appears that PER directly binds to CLK, whereas itself and TIM do not bind to Cycle (CYC). PER binding to CLK is in itself not sufficient for inhibition.

Literature References

PubMed ID	Title	Journal	Year
10409723	PER and TIM inhibit the DNA binding activity of a Drosophila CLOCK-CYC/dBMAL1 heterodimer without disrupting formation of the heterodimer: a basis for circadian transcription Bae, K, Edery, I, Lee, C	Mol Cell Biol	1999
10684876	dCLOCK is present in limiting amounts and likely mediates daily interactions between the dCLOCK-CYC transcription factor and the PER-TIM complex Bae, K, Hardin, PE, Edery, I, Lee, C	J Neurosci	2000
20159956	Dynamic PER repression mechanisms in the Drosophila circadian clock: from on-DNA to off-DNA Menet, JS, Rosbash, M, Abruzzi, KC, Desrochers, J, Rodriguez, J	Genes Dev	2010
17452449	A DOUBLETIME kinase binding domain on the Drosophila PERIOD protein is essential for its hyperphosphorylation, transcriptional repression, and circadian clock function Kim, EY, Hardin, PE, Edery, I, Ko, HW, Yu, W	Mol Cell Biol	2007
9808471	The Drosophila CLOCK protein undergoes daily rhythms in abundance, phosphorylation, and interactions with the PER-TIM complex Bae, K, Edery, I, Lee, C	Neuron	1998

Participants

Input

Output

Phospho TIM:DNA:CLK:CYC:Phospho (multi-site 2) PER:DCO [nucleoplasm] (Drosophila melanogaster)

Participates

as an event of

Transcription repression by PER and activation by PDP1 (Drosophila melanogaster)

Authored

Williams, MG (2010-03-08)

Reviewed

Edery, I (2010-07-06)

Created

Williams, MG (2010-03-08)

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