Cleavage of factor B to form classical C3 convertase

Stable Identifier
Reaction [transition]
Gallus gallus
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In mammals, C2 binds to surface-bound C4b to form the C4b:C2 complex, which makes C2 susceptible to cleavage by C1s or MASPs. C1s or MASPs cleaves C2 producing the C4b:C2b complex with the active serine protease component C2b. C4b:C2b remains on the target surface as a C3 convertase of the classical and lectin-mediated pathways (Laich A and Sim RB 2001; Wallis R et al 2007).

A homologue for C2 was not found in chickens (Barta O & Hubbert NL 1978). It was assumed that the role of C2 may be fulfilled by the chicken complement factor B-like protease (factor B), an evolutionary remnant of a common C2/factor B ancestor (Kjalke M et al. 1993; Salter-Cid L & Flajnik MF 1995). Chicken factor B-like protease (factor B) is a glycoprotein of 95 kDa. Activation of chicken serum complement with inulin cleaved factor B into an N-terminal Ba fragment of 37 kDa and a C-terminal Bb fragment of 60 kDa (Kjalke M et al. 1993). The whole protein and the two fragments were purified by affinity chromatography using mAb to chicken Ba or Bb followed by ion exchange chromatography (Kjalke M et al. 1993). Amino acid sequencing showed that the chicken factor B was cleaved at a site homologous to that cleaved in mammalian complement components B and C2 on activation. Limited tryptic digestion of the B-like protease generated fragments similar to Ba and Bb (Kjalke M et al. 1993). Comparison of chicken Ba to human and mouse C2b and Ba showed 42 to 45% sequence identity with respect to C2b fragments, and 46 to 49% sequence identity with respect to Ba fragments (Kjalke M et al. 1993). Thus, chicken factor B-like protease seemed to be equally related to mammalian complement components B and C2, and the B-like protease is thought to represent the present-day descendant of a common ancestral protein for mammalian B and C2 (Kjalke M et al. 1993).

Literature References
PubMed ID Title Journal Year
8409391 Structural analysis of chicken factor B-like protease and comparison with mammalian complement proteins factor B and C2

Welinder, KG, Koch, C, Kjalke, M

J Immunol 1993
17204478 Molecular interactions between MASP-2, C4, and C2 and their activation fragments leading to complement activation via the lectin pathway

Sim, RB, Dodds, AW, Mitchell, DA, Schwaeble, WJ, Reid, KB, Wallis, R

J Biol Chem 2007
11341920 Complement C4bC2 complex formation: an investigation by surface plasmon resonance

Sim, RB, Laich, A

Biochim Biophys Acta 2001
Catalyst Activity

serine-type endopeptidase activity of C4:factor B activator [plasma membrane]

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