Monoubiquitination of PCNA

Stable Identifier
Reaction [transition]
Gallus gallus
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RAD18 catalyzes mono ubiquitination of PCNA by its RING zinc finger domain, a modification central to DNA damage bypass and post replication repair. In contrast to yeast, studies performed in chicken DT40 cells reveal that RAD18 is essential for both post replication repair as well as homologous recombination repair. Because the repair of stalled replication forks is channeled into these two pathways, RAD18 minimizes the toxic effects of non homologous end joining (NHEJ) repair pathways. Thus, vertebrate RAD18 regulates the balance between NHEJ and HR.

Literature References
PubMed ID Title Journal Year
17242200 RAD18 and poly(ADP-ribose) polymerase independently suppress the access of nonhomologous end joining to double-strand breaks and facilitate homologous recombination-mediated repair

Saberi, A, Hochegger, H, Szuts, D, Lan, L, Yasui, A, Sale, JE, Taniguchi, Y, Murakawa, Y, Zeng, W, Yokomori, K, Helleday, T, Teraoka, H, Arakawa, H, Buerstedde, JM, Takeda, S

Mol Cell Biol 2007
16923963 Role for RAD18 in homologous recombination in DT40 cells

Sz?ts, D, Simpson, LJ, Kabani, S, Yamazoe, M, Sale, JE

Mol Cell Biol 2006
12374756 RAD18 and RAD54 cooperatively contribute to maintenance of genomic stability in vertebrate cells

Yamashita, YM, Okada, T, Matsusaka, T, Sonoda, E, Zhao, GY, Araki, K, Tateishi, S, Yamaizumi, M, Takeda, S

EMBO J 2002
Catalyst Activity

ubiquitin-protein transferase activity of RAD18 [nucleoplasm]

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