fructose 6-phosphate + ATP => fructose 2,6-bisphosphate + ADP

Stable Identifier
Reaction [transition]
Gallus gallus
Phosphorylation of fructose 6-phosphate to yield fructose 2,6-bisphosphate
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Cytosolic phosphofructokinase 2 (PFK2) catalyzes the irreversible reaction of fructose 6-phosphate and ATP to form fructose 2,6-bisphosphate and ADP. Fructose 2,6-bisphosphate is not an intermediate in the pathway from glucose 6-phosphate to pyruvate, but rather is a strong allosteric activator of the enzyme (PFK1) that catalyzes the synthesis of fructose 1,6-bisphosphate, and in mammalian systems regulation of fructose 2,6-bisphosphate levels plays a central role in regulating the rate of glycolysis. The chicken enzyme has been purified and shown to form a catalytically active dimer, though its role in the overall regulation of glycolysis is not fully worked out (Li et al. 1993; Van Schaftingen and Hers 1986). Two additional proteins, PFK2-like1 and 2, are predicted to have phosphofructokinase 2 activity based on OrthoMCL analysis of the ENSEMBL chicken gene set. Both of these are annotated here as monomers as no data concerning their biochemical properties are available.

Literature References
PubMed ID Title Journal Year
3019688 Purification and properties of phosphofructokinase 2/fructose 2,6-bisphosphatase from chicken liver and from pigeon muscle

Hers, HG, Van Schaftingen, E

Eur J Biochem 1986
7916593 Isolation of a cDNA for chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase

Li, L, Lange, AJ, Pilkis, SJ

Biochem Biophys Res Commun 1993
Catalyst Activity

6-phosphofructo-2-kinase activity of PFKFB4 dimer [cytosol]

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