Reactome | Insertion of correct bases opposite the lesion by POLH

Insertion of correct bases opposite the lesion by POLH

Stable Identifier

R-HSA-110317

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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Insertion of correct bases opposite the lesion by POLH

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DNA polymerase eta (POLH) correctly incorporates two adenine deoxyribonucleotides (dAMPs) opposite a TT-CPD (thymine-thymine cyclobutane pyrimidine dimer) lesion. POLH can bypass other types of lesions, such as AP sites and cisplatin-induced intrastrand cross-linked gunanines, preferentially incorporating dAMPs and dGMPs opposite the lesion. While POLH is accurate in translesion synthesis (TLS) across thymine dimers, POLH has a low fidelity in TLS across other DNA damage types and when copying undamaged DNA. One of the protective mechanisms against POLH-induced mutagenesis may be that POLH cannot continue chain elongation after an incorrect nucleotide is incorporated (Matsuda et al. 2000, Masutani et al. 2000).

Literature References

PubMed ID	Title	Journal	Year
10856253	Mechanisms of accurate translesion synthesis by human DNA polymerase eta. Kusumoto, R, Iwai, S, Masutani, C, Hanaoka, F	EMBO J	2000
10801132	Low fidelity DNA synthesis by human DNA polymerase-eta Kunkel, TA, Masutani, C, Matsuda, T, Bebenek, K, Hanaoka, F	Nature	2000