FEN1 bound to PCNA and APEX1 cleaves flap ssDNA

Stable Identifier
R-HSA-110363
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
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FEN1 bound to PCNA and APEX1 cleaves flap ssDNA
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PCNA-bound FEN1 acts as a flap endonuclease to cleave the displaced single strand DNA (flap) containing the oxidatively damaged AP (apurinic/apyrimidinic) dideoxyribose residue (5'ddRP) that could not be excised by POLB. Interaction between FEN1 and PCNA or FEN1 and APE1 facilitate cleavage of flap structures (Klungland and Lindahl 1997, Matsumoto et al. 1999, Dianov et al. 1999).
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Catalyst Activity

5'-flap endonuclease activity of APEX1:FEN1:PCNA:POLD,POLE:RPA:RFC:SSB(3'poly-dNMP-displaced 5'ddRP flap)-dsDNA [nucleoplasm]

Physical Entity
APEX1:FEN1:PCNA:POLD,POLE:RPA:RFC:SSB(3'poly-dNMP-displaced 5'ddRP flap)-dsDNA [nucleoplasm] (Homo sapiens)
Activity
5'-flap endonuclease activity (GO:0017108)
Authored
Matthews, L  (2004-01-29)
Reviewed
Borowiec, JA  (2014-12-22)
Created
Matthews, L  (2004-01-29)
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