Reactome | POLD,POLE-mediated DNA strand displacement synthesis

POLD,POLE-mediated DNA strand displacement synthesis

Stable Identifier

R-HSA-110368

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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POLD,POLE-mediated DNA strand displacement synthesis

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Polymerase delta complex (POLD) or polymerase epsilon complex (POLE) bound to PCNA-associated replication complex that also includes RPA and RFC complexes, catalyzes DNA strand displacement synthesis in a reaction facilitated by FEN1 and APEX1. POLD or POLE extend the 3' end of the single strand break (SSB) by adding up to 10 nucleotides. The 5' end of the SSB, which contains the oxidatively damaged AP (apurinic/apyrimidinic) dideoxyribose phosphate residue (5'ddRP) that could not be excised by POLB, is displaced as a flap structure by the joint action of FEN1 and POLD or POLE (Klungland and Lindahl 1997, Stucki et al. 1998, Dianov et al. 1999, Podlutsky et al. 2001, Ranalli et al. 2002).

Literature References

PubMed ID	Title	Journal	Year
10460157	Replication protein A stimulates proliferating cell nuclear antigen-dependent repair of abasic sites in DNA by human cell extracts. Bohr, VA, Kenny, MK, Dianov, GL, Jensen, BR	Biochemistry	1999
9780000	Mammalian base excision repair by DNA polymerases delta and epsilon Dogliotti, E, Hübscher, U, Wilson, SH, Fortini, P, Pascucci, B, Parlanti, E, Stucki, M	Oncogene	1998
11250913	Human DNA polymerase beta initiates DNA synthesis during long-patch repair of reduced AP sites in DNA. Podust, VN, Podlutsky, AJ, Dianova, II, Bohr, VA, Dianov, GL	EMBO J	2001
12200445	AP endonuclease 1 coordinates flap endonuclease 1 and DNA ligase I activity in long patch base excision repair. Ranalli, TA, Bambara, RA, Tom, S	J Biol Chem	2002
9214649	Second pathway for completion of human DNA base excision-repair: reconstitution with purified proteins and requirement for DNase IV (FEN1). Lindahl, T, Klungland, A	EMBO J	1997