POLB incorporates the first 3' dNMP and displaces 5'ddRP at SSB site

Stable Identifier
R-HSA-111253
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
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POLB incorporates the first 3' dNMP and displaces 5'ddRP at SSB site
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DNA polymerase beta (POLB) cannot excise oxidatively damaged 5' AP (apurinic/apyrimidinic) dideoxyribose residue (5'ddRP) at the APEX1-generated single strand break (SSB). Instead, POLB incorporates the first nucleotide (dNMP) at the 3' end of the SSB, which displaces 5'ddRP (Podlutsky et al. 2001).
Literature References
PubMed ID Title Journal Year
11250913 Human DNA polymerase beta initiates DNA synthesis during long-patch repair of reduced AP sites in DNA.

Podust, VN, Podlutsky, AJ, Dianova, II, Bohr, VA, Dianov, GL

EMBO J 2001
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Catalyst Activity

DNA-directed DNA polymerase activity of POLB:APEX1:SSB(5'ddRP)-dsDNA [nucleoplasm]

Physical Entity
POLB:APEX1:SSB(5'ddRP)-dsDNA [nucleoplasm] (Homo sapiens)
Activity
DNA-directed DNA polymerase activity (GO:0003887)
Orthologous Events
POLB incorporates the first 3' dNMP and displaces 5'ddRP at SSB site (Bos taurus)
POLB incorporates the first 3' dNMP and displaces 5'ddRP at SSB site (Canis familiaris)
POLB incorporates the first 3' dNMP and displaces 5'ddRP at SSB site (Danio rerio)
POLB incorporates the first 3' dNMP and displaces 5'ddRP at SSB site (Dictyostelium discoideum)
POLB incorporates the first 3' dNMP and displaces 5'ddRP at SSB site (Mus musculus)
POLB incorporates the first 3' dNMP and displaces 5'ddRP at SSB site (Rattus norvegicus)
POLB incorporates the first 3' dNMP and displaces 5'ddRP at SSB site (Sus scrofa)
POLB incorporates the first 3' dNMP and displaces 5'ddRP at SSB site (Xenopus tropicalis)
Authored
Matthews, L  (2004-02-03)
Reviewed
Borowiec, JA  (2014-12-22)
Created
Matthews, L  (2004-02-09)
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