Reactome | Addition of nucleotides between position +11 and +30

Addition of nucleotides between position +11 and +30

Stable Identifier

R-HSA-111264

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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Addition of nucleotides between position +11 and +30

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RNA polymerase II transcription complexes are susceptible to transcriptional stalling and arrest, when extending nascent transcripts to 30-nt. This susceptibility depends on presence on down-stream DNA, the particular DNA-sequence of the template and presence of transcription factors. Transcription factor TFIIH remains associated to the RNA pol II elongation complex until position +30. At this stage transcription elongation factor TFIIS can rescue stalled transcription elongation complexes. The transcription bubble varies between 13- and 22-nt in size.

Literature References

PubMed ID	Title	Journal	Year
7601352	Recycling of the general transcription factors during RNA polymerase II transcription. Zawel, L, Kumar, KP, Reinberg, D	Genes Dev	1995
11486021	Promoter clearance by RNA polymerase II is an extended, multistep process strongly affected by sequence. McKean, D, Pal, M, Luse, DS	Mol Cell Biol	2001
9353262	Promoter escape by RNA polymerase II. Formation of an escape-competent transcriptional intermediate is a prerequisite for exit of polymerase from the promoter. Tan, S, Conaway, JW, Dvir, A, Conaway, RC	J Biol Chem	1997
11433015	Analysis of the open region of RNA polymerase II transcription complexes in the early phase of elongation. Fiedler, U, Timmers, HT	Nucleic Acids Res	2001