3' PPT-primed initiation of plus-strand DNA synthesis

Stable Identifier
R-HSA-164513
Type
Reaction [transition]
Species
Homo sapiens
Related Species
Human immunodeficiency virus 1
Compartment
cytosol
Synonyms
ReviewStatus
5/5
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3' PPT-primed initiation of plus-strand DNA synthesis
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HIV-1 genomic RNA contains a centrally located PPT (cPPT) within the pol gene that, like 3'PPT, is spared by RNase H during minus-strand DNA synthesis and persists to prime plus-strand DNA synthesis. This ribonucleotide primes the synthesis of a plus-strand DNA extending through the U3 and R regions of the HIV sequence and terminating in the PBS region (the tRNA primer-binding site). This DNA segment is known as plus-strand strong-stop DNA (+sssDNA) (Telesnitsky and Goff 1997; Pullen et al. 1993; Huber and Richardson 1990). cPPT priming is important for efficient viral replication (Alizon et al. 1992; Rausch and Le Grice 2004). Several features of cPPT priming in vivo remain to be clarified.
Literature References
PubMed ID Title Journal Year
10723025 The HIV-1 reverse transcription (RT) process as target for RT inhibitors

De Clercq, E, Anne, J, Jonckheere, H

Med Res Rev 2000
7681062 The sequence features important for plus strand priming by human immunodeficiency virus type 1 reverse transcriptase

Pullen, KA, Champoux, JJ, Rattray, AJ

J Biol Chem 1993
1693920 Processing of the primer for plus strand DNA synthesis by human immunodeficiency virus 1 reverse transcriptase

Huber, HE, Richardson, CC

J Biol Chem 1990
  Retroviruses

Varmus, HE, Hughes, SH, Coffin, JM

  1997
Participants
Input
Output
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as an event of
Catalyst Activity

DNA-directed DNA polymerase activity of RTC with extending minus strand DNA [cytosol]

Physical Entity
RTC with extending minus strand DNA [cytosol] (Homo sapiens)
Activity
DNA-directed DNA polymerase activity (GO:0003887)
Disease
Name Identifier Synonyms
Human immunodeficiency virus infectious disease DOID:526 HIV infection
Authored
D'Eustachio, P  (2006-05-19)
Gopinathrao, G  (2006-05-19)
Reviewed
Hughes, SH  (2006-10-31)
Created
D'Eustachio, P  (2005-05-24)
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