Terminal (3' end) cleavage of viral DNA

Stable Identifier
R-HSA-164522
Type
Reaction [transition]
Species
Homo sapiens
Related Species
Human immunodeficiency virus 1
Compartment
cytosol
ReviewStatus
5/5
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Terminal (3' end) cleavage of viral DNA
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Prior to integration, two nucleotides are removed from each 3' end of the linear viral DNA, thereby exposing recessed 3' hydroxyls. This reaction may serve to remove heterogenous extra bases from the viral DNA end, and to stabilize the IN-DNA complex. The chemistry of cleavage is a simple hydrolysis by single-step transesterification.
Literature References
PubMed ID Title Journal Year
2539592 Retroviral integration: structure of the initial covalent product and its precursor, and a role for the viral IN protein

Brown, PO, Varmus, HE, Bowerman, B, Bishop, JM

Proc Natl Acad Sci U S A 1989
  Retroviruses

Varmus, HE, Hughes, SH, Coffin, JM

  1997
8883604 HIV cDNA integration: molecular biology and inhibitor development

Bushman, FD, Farnet, CM

AIDS 1996
  Genetic Engineering: Principles and Methods

Setlow, JK

  1998
Participants
Input
Output
Participates
as an event of
Disease
Name Identifier Synonyms
Human immunodeficiency virus infectious disease DOID:526 HIV infection
Authored
D'Eustachio, P  (2006-05-19)
Gopinathrao, G  (2006-05-19)
Reviewed
Bushman, FD  (2006-10-31)
Created
D'Eustachio, P  (2005-05-24)
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