Addition of the fourth nucleotide on the nascent HIV-1 transcript: Second Transition

Stable Identifier
R-HSA-167113
Type
Reaction [omitted]
Species
Homo sapiens
Related Species
Human immunodeficiency virus 1
Compartment
ReviewStatus
5/5
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Formation of the third phosphodiester bond creates a 4-nt product. This commits the initiation complex to promoter escape. The short 4-nt transcript is still loosely associated with the RNA polymerase II initiation complex and can dissociate to yield abortive products, which are not further extended. Inhibition of ATP-hydrolysis by TFIIH does not lead to collapse of the open region any longer. The transcription complex has lost the sensitivity to single-stranded oligo-nucleotide inhibition. However, ATP-hydrolysis and TFIIH are required for efficient promoter escape. The open region ("transcription bubble") expands concomitant with the site of RNA-extension. In this case this region spans positions -9 to +4.
Literature References
PubMed ID Title Journal Year
11784853 Translocation after synthesis of a four-nucleotide RNA commits RNA polymerase II to promoter escape.

Goodrich, JA, Kugel, JF

Mol Cell Biol 2002
9405375 Three transitions in the RNA polymerase II transcription complex during initiation.

Fiedler, U, Timmers, HT, Holstege, FC

EMBO J 1998
Participants
Participates
Catalyst Activity

DNA-directed 5'-3' RNA polymerase activity of RNA Polymerase II (unphosphorylated):TFIIF complex [nucleoplasm]

Inferred From
Disease
Name Identifier Synonyms
Human immunodeficiency virus infectious disease DOID:526 HIV infection
Authored
Created
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