CHUK:IKBKB:IKBKG [cytosol]

Stable Identifier
R-HSA-168113
Type
Complex
Species
Homo sapiens
Compartment
cytosol
Synonyms
IKKA:IKBKB:IKBKG, IKK complex, Inhibitor of KappaB kinase (IKK) Complex
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General
Co-immunoprecipitation studies and size exclusion chromatography analysis indicate that the high molecular weight (around 700 to 900 kDa) IKK complex is composed of two kinase subunits (IKK1/CHUK/IKBKA and/or IKK2/IKBKB/IKKB) bound to a regulatory gamma subunit (IKBKG/NEMO) (Rothwarf DMet al. 1998; Krappmann D et al. 2000; Miller BS & Zandi E 2001). Variants of the IKK complex containing IKBKA or IKBKB homodimers associated with NEMO may also exist. Crystallographic and quantitative analyses of the binding interactions between N-terminal NEMO and C-terminal IKBKB fragments showed that IKBKB dimers would interact with NEMO dimers resulting in 2:2 stoichiometry (Rushe M et al. 2008). Chemical cross-linking and equilibrium sedimentation analyses of IKBKG (NEMO) suggest a tetrameric oligomerization (dimers of dimers) (Tegethoff S et al. 2003). The tetrameric NEMO could sequester four kinase molecules, yielding an 2xIKBKA:2xIKBKB:4xNEMO stoichiometry (Tegethoff S et al. 2003). The above data suggest that the core IKK complex consists of an IKBKA:IKBKB heterodimer associated with an IKBKG dimer or higher oligomeric assemblies. However, the exact stoichiometry of the IKK complex remains unclear.
Literature References
PubMed ID Title Journal Year
20434986 NLRC5 negatively regulates the NF-kappaB and type I interferon signaling pathways

Shen, P, Cui, J, Wang, HY, Hong, J, Wang, RF, Xia, X, Zhu, L, Ji, J, Chen, ZJ, Legras, X, Zheng, S

Cell 2010
9751060 IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex

Karin, M, Rothwarf, DM, Natoli, G, Zandi, E

Nature 1998
10893415 The I kappa B kinase (IKK) complex is tripartite and contains IKK gamma but not IKAP as a regular component

Hatada, EN, Tegethoff, S, Baeuerle, PA, Scheidereit, C, Klippel, A, Giese, K, Krappmann, D, Li, J

J. Biol. Chem. 2000
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Inferred To
CHUK:IKBKB:IKBKG [cytosol] (Bos taurus)
CHUK:IKBKB:IKBKG [cytosol] (Canis familiaris)
CHUK:IKBKB:IKBKG [cytosol] (Drosophila melanogaster)
CHUK:IKBKB:IKBKG [cytosol] (Mus musculus)
CHUK:IKBKB:IKBKG [cytosol] (Rattus norvegicus)
CHUK:IKBKB:IKBKG [cytosol] (Sus scrofa)
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