Reactome | CAK phosphorylates CDK1 bound to CCNA

CAK phosphorylates CDK1 bound to CCNA

Stable Identifier

R-HSA-170087

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

Synonyms

CAK-mediated phosphorylation of Cyclin A:Cdc2 complexes

ReviewStatus

5/5

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Full activity of most CDKs is dependent on CAK mediated phosphorylation at a conserved residue (Thr161 in CDK1, also known as Cdc2). This modification is thought to improve substrate binding. High affinity binding of Cyclin A within the Cyclin A:Cdc2 complex requires this phosphorylation (Desai et al 1995, Merrick et al. 2008). CAK is unable to phosphorylate monomeric CDK1 and only phosphorylates it when it is bound to cyclin A or cyclin B (Merrick et al. 2008). Using two-dimensional gel electrophoresis of protein extracts of cycling human cells, it was shown that CDK1 phosphorylated at both T14 nad T161 can be co-immunoprecipitated with CCNA2 before mitosis (Coulonval et al. 2011).

Literature References

PubMed ID	Title	Journal	Year
21900495	Coupling of T161 and T14 phosphorylations protects cyclin B-CDK1 from premature activation Kooken, H, Coulonval, K, Roger, PP	Mol Biol Cell	2011
7799941	Effects of phosphorylation by CAK on cyclin binding by CDC2 and CDK2. Wessling, HC, Desai, D, Fisher, RP, Morgan, DO	Mol Cell Biol	1995
19061641	Distinct activation pathways confer cyclin-binding specificity on Cdk1 and Cdk2 in human cells Shokat, KM, Zhang, C, Merrick, KA, Larochelle, S, Allen, JJ, Fisher, RP	Mol Cell	2008