PI(4,5)P2 is hydrolysed to I(1,4,5)P3 and DAG by tethered PLC[1] at the plasma membrane

Stable Identifier
Reaction [transition]
Homo sapiens
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A group of phospholipase C proteins (“PLC(degh)”) bind to the plasma membrane via their PH domains. These phospholipases hydrolyse phosphatidylinositol 4,5 bisphosphate (PI(4,5)P2) to inositol 1,4,5 trisphosphate (I(1,4,5)P3) and diacylglycerol (DAG). The C2 domains of the enzymes bind to PI(4,5)P2 at the membrane.

The phospholipase C isoforms involved and their corresponding literature references are: phosphoinositide phospholipase C delta-1(PLCD1) (Cheng et al. 1995); epsilon-1 (PLCE1) (Song et al. 2001, Lopez et al. 2001); delta-3 (PLCD3) (Pawelczyk & Matecki 1997); gamma-1 (PLCG1) (Harita et al. 2009, Baldassare et al. 1989); gamma-2 (PLCG2) (Banno et al. 1988); eta-1 (PLCH1) (Hwang et al. 2005); and eta-2 (PLCH2) (Zhou et al 2005).
Literature References
PubMed ID Title Journal Year
2841328 Purification and characterization of membrane-bound phospholipase C specific for phosphoinositides from human platelets

Nozawa, Y, Yada, Y, Banno, Y

J Biol Chem 1988
15702972 Molecular cloning and characterization of a novel phospholipase C, PLC-eta

Suh, PG, Shin, KJ, Oh, YS, Ryu, SH, Kim, H, Hwang, JI

Biochem J 2005
9360711 Expression, purification and kinetic properties of human recombinant phospholipase C delta 3

Matecki, A, Pawe?czyk, T

Acta Biochim Pol 1997
16107206 Molecular cloning and characterization of PLC-eta2

Wing, MR, Zhou, Y, Sondek, J, Harden, TK

Biochem J 2005
11022048 Regulation of a novel human phospholipase C, PLCepsilon, through membrane targeting by Ras

Shibatohge, M, Satoh, T, Song, C, Kariyai, K, Hu, CD, Wu, D, Masago, M, Kataoka, T, Yamawaki-Kataoka, Y

J Biol Chem 2001
2550068 Isolation and characterization of one soluble and two membrane-associated forms of phosphoinositide-specific phospholipase C from human platelets

Fisher, GJ, Henderson, PA, Baldassare, JJ

Biochemistry 1989
19179337 Phosphorylation of Nephrin Triggers Ca2+ Signaling by Recruitment and Activation of Phospholipase C-{gamma}1

Kurihara, H, Igarashi, T, Harita, Y, Ohta, S, Tezuka, T, Hattori, S, Kosako, H, Ohsawa, I, Sekine, T

J Biol Chem 2009
7890667 Cloning and identification of amino acid residues of human phospholipase C delta 1 essential for catalysis

King, K, Chen, CL, Jiang, MJ, Cheng, HF, Wong, LP, Lomasney, JW, Liu, SM

J Biol Chem 1995
11022047 A novel bifunctional phospholipase c that is regulated by Galpha 12 and stimulates the Ras/mitogen-activated protein kinase pathway

Ding, J, Mak, EC, Hamm, HE, Lomasney, JW, Lopez, I

J Biol Chem 2001
Catalyst Activity

phosphatidylinositol phospholipase C activity of PLCdegh [plasma membrane]

Orthologous Events
Cross References
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