Cleavage of DNA by DFF40

Stable Identifier
Reaction [transition]
Homo sapiens
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The DFF40 cleaves DNA substrates to generate fragments possessing ends with 5’-phosphate and 3’-hydroxyl groups, and generates exclusively double strand breaks (primarily blunt ends). It has some sequence preferences on naked DNA substrates and prefers purine/pyrimidine blocks with rotational symmetry (Widlak et al., 2000). DFF is both a deoxyribonucleotide-specific and a double-strand-specific endonuclease (Hanus et al., 2008).

Literature References
PubMed ID Title Journal Year
10713148 Cleavage preferences of the apoptotic endonuclease DFF40 (caspase-activated DNase or nuclease) on naked DNA and chromatin substrates

Li, P, Wang, X, Widlak, P, Garrard, WT

J Biol Chem 2000
18283539 The major apoptotic endonuclease DFF40/CAD is a deoxyribose-specific and double-strand-specific enzyme

Hanus, J, Kalinowska-Herok, M, Widlak, P

Apoptosis 2008
Catalyst Activity

deoxyribonuclease activity of DFF40 associated with chromatin [nucleoplasm]

This event is regulated