Reactome | Formation of TRAP coactivator complex

Formation of TRAP coactivator complex

Stable Identifier

R-HSA-212380

Type

Reaction [binding]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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TRAP co-activator complex and assembly

The TRAP co-activator complex is a subset of 18 proteins from the set of at least 31 Mediator proteins that, in different combinations and in different contexts, form specific co-activator or "Adapter" complexes in human cells. These complexes bridge between the basal transcription factors (including Pol II) and tissue-specific transcription factors (TFs) bound to sites within upstream Proximal Promoter regions or distal Enhancer regions (reviewed in Maston, 2006 and Naar, 2001).

The TRAP complex was originally identified and named as a co-activator complex associated with the Thyroid Hormone Receptor member of the nuclear receptor family of transcription factors (Yuan, 1998). It was later determined that many of the components of the TRAP complex are also in the DRIP complex, and in the ARC complex.

The TRAP complex contains the following 14 proteins, which also are common to the DRIP and ARC complexes: MED1, MED4, MED6, MED7, MED10, MED12, MED13, MED14, MED16, MED17, MED23, MED24, CDK8, CycC.

The TRAP complex also contains 4 additional components, which are now called: MED20, MED27, MED30, and MED 31 in the unified nomenclature scheme (Bourbon, 2004).

In addition, these various transcription co-activator proteins identified in mammalian cells were found to be the orthologues or homologues of the Mediator complex proteins in yeast, first identified by Kornberg and colleagues (Kelleher, 1990). The unified nomenclature system for these adapter / co-activator proteins now labels them Mediator 1 through Mediator 31 (Bourbon, 2004).

The order of addition of the TRAP proteins during complex assembly is not fully determined, and may vary in different cell contexts. Therefore, TRAP co-activator complex assembly is represented as a single reaction event, in which all 18 components assemble simultaneously into the TRAP co-activator complex.

Literature References

PubMed ID	Title	Journal	Year
9637681	A novel protein complex that interacts with the vitamin D3 receptor in a ligand-dependent manner and enhances VDR transactivation in a cell-free system Suldan, Z, Freedman, LP, Chang, CP, Ward, J, Tempst, P, Rachez, C, Erdjument-Bromage, H, Burakov, D	Genes Dev	1998
10235266	Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex Bromleigh, V, Gamble, M, Suldan, Z, Lemon, BD, Freedman, LP, Tempst, P, Näär, AM, Rachez, C, Erdjument-Bromage, H	Nature	1999
2163759	A novel mediator between activator proteins and the RNA polymerase II transcription apparatus Kornberg, RD, Kelleher RJ, 3rd, Flanagan, PM	Cell	1990
15175151	A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II Naar, AM, Roeder, RG, Sadowski, I, Sipiczki, M, Carey, M, He, X, Bjorklund, S, Hinnebusch, AG, Jaehning, JA, Strich, R, Gustafsson, CM, Holstege, FC, Fukasawa, T, Leutz, A, Nasmyth, K, Ronne, H, Emmons, SW, Carlson, M, Lis, JT, Tuck, S, Bourbon, HM, Conaway, RC, Kuras, L, Parvin, JD, Conaway, JW, Winston, F, Sternberg, PW, Svejstrup, JQ, Freedman, LP, Struhl, K, Reinberg, D, Fondell, JD, Herman, PK, Kornberg, RD, Han, M, Holmberg, S, Sakurai, H, Meisterernest, M, Blackwell, TK, Kim, YJ, Stillman, DJ, Berk, AJ, Ansari, AZ, Aguilera, A, Ptashne, M, Borggrefe, T, Asturias, FJ	Mol Cell	2004
9653119	The TRAP220 component of a thyroid hormone receptor- associated protein (TRAP) coactivator complex interacts directly with nuclear receptors in a ligand-dependent fashion Yuan, CX, Fondell, JD, Fu, ZY, Ito, M, Roeder, RG	Proc Natl Acad Sci U S A	1998