Arachidonic acid is oxidised to 15S-HpETE by ALOX15/15B

Stable Identifier
Reaction [transition]
Homo sapiens
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Arachidonate 15-lipoxygenase (ALOX15) (Gulliksson et al. 2007, Kuhn et al. 1993, Izumi et al. 1991) and arachidonate 15-lipoxygenase B (ALOX15B) (Tang et al. 2002, Wecksler et al. 2008) are lipid peroxidising enzymes mainly expressed in airway epithelial cells, eosinophils, reticulocytes and in macrophages. They insert molecular oxygen at C-6 from the omega-end of arachidonic acid with formation of the unstable intermediate 15S-hydroperoxyeicosatetraenoic acid (15S-HpETE) which can be further converted, enzymatically or non-enzymatically, to 15S-hydroxyeicosatetraenoic acid (15S-HETE).
Literature References
PubMed ID Title Journal Year
18570379 Substrate specificity changes for human reticulocyte and epithelial 15-lipoxygenases reveal allosteric product regulation

Kenyon, V, Wecksler, AT, Deschamps, JD, Holman, TR

Biochemistry 2008
1662607 Purification of two forms of arachidonate 15-lipoxygenase from human leukocytes

Jörnvall, H, Rådmark, O, Izumi, T, Samuelsson, B

Eur J Biochem 1991
8334154 Overexpression, purification and characterization of human recombinant 15-lipoxygenase

Bursztyn-Pettegrew, H, Chow, J, Baecker, P, Grunberger, D, Kühn, H, Barnett, J, Sigal, E, Chan, H, Nguyen, B

Biochim Biophys Acta 1993
11839751 Evidence that arachidonate 15-lipoxygenase 2 is a negative cell cycle regulator in normal prostate epithelial cells

Liu, J, Traag, J, Zhau, HE, Tang, DG, Shen, J, Klein, RD, Maldonado, CJ, Tang, S, Chung, LW, Yang, P, Newman, RA, Chandra, D, Bhatia, B, Fischer, SM, Chopra, D

J Biol Chem 2002
17662651 Expression of 15-lipoxygenase type-1 in human mast cells

Kumlin, M, Nilsson, G, Brunnström, A, Harvima, I, Dahlén, B, Backman, L, Gulliksson, M, Claesson, HE, Johannesson, M

Biochim Biophys Acta 2007
Catalyst Activity

arachidonate 15-lipoxygenase activity of ALOX15/15B [cytosol]

Orthologous Events
Cross References
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