Conjugation of SUMO3 to UBA2:SAE1

Stable Identifier
R-HSA-2993781
Type
Reaction [transition]
Species
Homo sapiens
Compartment
ReviewStatus
5/5
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The UBA2:SAE1 complex catalyzes the formation of a thioester bond between SUMO3 and cysteine-173 of UBA2 (Tatham et al. 2001, Werner et al. 2009). ATP reacts with the C-terminal glycine residue of SUMO3 to yield pyrophosphate and a transient intermediate, SUMO3 adenylate, which then reacts with the thiol group of the cysteine residue on UBA2.
Literature References
PubMed ID Title Journal Year
11451954 Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9

Vaughan, OA, Tatham, MH, Botting, CH, Naismith, JH, Desterro, JM, Jaffray, E, Hay, RT

J. Biol. Chem. 2001
19107418 Performing in vitro sumoylation reactions using recombinant enzymes

Moutty, MC, Melchior, F, Möller, U, Werner, A

Methods Mol. Biol. 2009
Participants
Participates
Catalyst Activity

SUMO activating enzyme activity of UBA2:SAE1 [nucleoplasm]

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