Reactome | Phosphorylation of nephrin by FYN

Phosphorylation of nephrin by FYN

Stable Identifier

R-HSA-373747

Type

Reaction [transition]

Species

Homo sapiens

Compartment

plasma membrane

ReviewStatus

5/5

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Nephrin has eight putative phosphorylation sites, which nicely match with substrate sites for the Src kinase family. Nephrin, is tyrosine phosphorylated by the Src family tyrosine kinase, Fyn in developing and injured podocytes. Phosphorylation of three of these tyrosines (1176, 1193 and 1217) results in the formation of a preferred binding motif (YDXV) for the SH2 domain of the adaptor protein NCK, which is one of the bricks linking Nephrin to the cytoskeleton. Phosphorylation of tyrosine 1158 results in a binding motif for the p85 regulatory subunit of PI3K.

Literature References

PubMed ID	Title	Journal	Year
18033240	Nephrin mediates actin reorganization via phosphoinositide 3-kinase in podocytes Kawachi, H, Li, H, Zhu, J, Lemay, S, Aoudjit, L, Takano, T, Sun, N	Kidney Int	2008
12846735	Clustering-induced tyrosine phosphorylation of nephrin by Src family kinases Pikkarainen, T, Liu, XL, Reponen, P, Kilpelainen, P, Tryggvason, K, Ruotsalainen, V, Lahdenpera, J	Kidney Int	2003
16543952	Nephrin ectodomain engagement results in Src kinase activation, nephrin phosphorylation, Nck recruitment, and actin polymerization Soofi, A, Holzman, LB, Patrie, K, Verma, R, Kovari, I, Nihalani, D	J Clin Invest	2006
15579503	SRC-family kinase Fyn phosphorylates the cytoplasmic domain of nephrin and modulates its interaction with podocin Kawachi, H, Li, H, Lemay, S, Aoudjit, L, Takano, T	J Am Soc Nephrol	2004
12668668	Fyn binds to and phosphorylates the kidney slit diaphragm component Nephrin Kunkel, R, Wharram, B, Holzman, LB, Verma, R, Kovari, I, Killen, P, Wiggins, RC, Nihalani, D, Wary, KK	J Biol Chem	2003