CHL1 accumulates in the presynaptic plasma membrane and it recruits heat shock cognate 71 kDa protein (HSP7C/HSC70), in an ADP dependent manner. HSC70 interacts with the intracellular domain of CHL1. Upon synapse activation, CHL1 along with HSC70 is endocytosed and is targeted to synaptic vesicles (SVs). In synapses, HSC70 regulates uncoating of synaptic vesicles (SVs) in the clathrin-dependent recycling pathway (Leshchyns'ka et al. 2006, Zinsmaier & Bronk 2001).