Reactome | Phosphorylation of HSF1 at Ser230 induces transactivation

Phosphorylation of HSF1 at Ser230 induces transactivation

Stable Identifier

R-HSA-5082387

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

Locations in the PathwayBrowser

Expand all

General

SBML | | PDF

SVG | | PPTX | SBGN

Phosphorylation of HSF1 at Ser230 induces transactivation

Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

The transcriptional activity of HSF1 has been shown to be controlled by the regulatory domain composed of amino acids 221-310 (Green M et al. 1995; Zuo J et al. 1995; Newton EM et al., 1996). Ser230 is located in this regulatory domain of HSF1 and is constitutively and stress-inducibly phosphorylated (Holmberg CI et al. 2001). Analyses with phosphopeptide-specific antibody and site-directed mutagenesis revealed that phosphorylation at Ser230 enhanced the inducible HSF1 transcriptional activity in heat-shocked human K562 erythroleukemia and HeLa cells (Holmberg CI et al 2001). Active calcium/calmodulin-dependent protein kinase II (CaMKII) was shown to phosphorylate HSF1 at Ser230 in vitro. Moreover, CaMKII enhanced heat-induced tranactivating capacity of HSF1 and the level of endogenous Ser230 phosphorylation in K562 cells transfected with active CaMKII together with a CAT reporter plasmid containing the proximal HSE of human hsp70 promoter. Thus, CaMKII signaling may be involved in the positive regulation of HSF1-mediated transactivation. However, the possibility that other protein kinases might also phosphorylate Ser230 in vivo should not be excluded (Holmberg CI et al 2001).

Literature References

PubMed ID	Title	Journal	Year
15760475	Analysis of phosphorylation of human heat shock factor 1 in cells experiencing a stress Lane, WS, Voellmy, R, Guettouche, T, Boellmann, F	BMC Biochem.	2005
11447121	Phosphorylation of serine 230 promotes inducible transcriptional activity of heat shock factor 1 Rantanen, JO, Holmberg, CI, Sistonen, L, Meinander, A, Morrice, N, Morimoto, RI, Kallio, M, Eriksson, JE, Mikhailov, A, MacKintosh, C, Hietakangas, V, Hellman, J	EMBO J.	2001