pagA(197-794):ANTRX2 oligomer transports cya and lef (target cell endosome to cytosol)

Stable Identifier
Reaction [omitted]
Homo sapiens
Related Species
Bacillus anthracis
Locations in the PathwayBrowser
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

Through the action of vacuolar ATPase the pH of the target cell early endosome is lowered. In this environment, paga (PA63, Anthrax protective antigen, large fragment) dissociates from its receptor and forms an oligomeric channel in the endosome membrane through which the anthrax cya (EF, edema factor) and lef (LF, lethal factor) pass (Milne et al. 1994). Entry of cya and lef into the target cell cytosol is thought to be mediated by back fusion of intraluminal vesicles with the late endosomal membrane and to be positively regulated by PDCD6IP / ALIX protein (Abrami et al. 2004).

Literature References
PubMed ID Title Journal Year
15337774 Membrane insertion of anthrax protective antigen and cytoplasmic delivery of lethal factor occur at different stages of the endocytic pathway

Leppla, SH, Parton, RG, Lindsay, M, van der Goot, FG, Abrami, L

J. Cell Biol. 2004
8051159 Anthrax protective antigen forms oligomers during intoxication of mammalian cells

Milne, JC, Hanna, PC, Collier, RJ, Wall, JS, Furlong, D

J. Biol. Chem. 1994
Catalyst Activity

protein transmembrane transporter activity of cya:lef:(pagA(197-794):ANTXR2 oligomer) [endosome membrane]

This event is regulated
Positively by