Reactome | B-WICH complex binds rDNA promoter

B-WICH complex binds rDNA promoter

Stable Identifier

R-HSA-5250947

Type

Reaction [binding]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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Nuclear myosin 1 is positively regulated by the glycogen synthase kinase (GSK3beta) by direct phosphorylation (Sarshad et al. 2014). NM1 phosphorylation is a requirement for NM1 to bind to the chromatin. In the absence of GSK3beta dependent phosphorylation NM1 is polyubiquitinated by the E3 ligase UBR5 and degraded by the proteasome and rRNA synthesis is not activated (Sarshad et al. 2014). Active rRNA genes are bound by the B‑WICH multiprotein complex (Cavellan et al. 2006, Percipalle et al. 2006). B-WICH binds the promoter region of the gene (Percipalle et al. 2006, Sarshad et al. 2013). The MYO1C component of the B-WICH complex binds chromatin and interacts with SMARCA5. Binding causes 200 bp of chromatin at the promoter to adopt a more open configuration and contributes to epigenetic modifications compatible with transcription activation (Vintermist et al. 2011, Sarshad et al. 2013). At the rRNA gene promoter the SMARCA5-MYOIC interaction is excluded when MYOIC interacts with actin in complex with RNA polymerase I (Sarshad et al. 2013). Binding of MYOIC to chromatin is regulated by GSK3beta-dependent phosphorylation that targets the MYOIC chromatin binding domain (Sarshad et al. 2014). Binding of MYOIC to the RNA polymerase I is partly mediated via phosphorylated TIF1A (Philimonenko et al. 2004). Binding of B‑WICH to rRNA genes requires MYOIC to be recruited to active rRNA genes and this mechanism appears to be a requirement to activate and maintain transcription by RNA polymerase I (Percipalle et al. 2006, Sarshad et al. 2013, Sarshad et al. 2014, reviewed in Sarshad and Percipalle 2014).

Literature References

PubMed ID	Title	Journal	Year
16603771	The WSTF-SNF2h chromatin remodeling complex interacts with several nuclear proteins in transcription Farrants, AK, Cavellán, E, Percipalle, P, Asp, P	J. Biol. Chem.	2006
16514417	The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription Farrants, AK, Scheer, U, Cavellán, E, Percipalle, P, Voit, R, Fomproix, N, Thyberg, J, Reimer, G, Krüger, T, Grummt, I	EMBO Rep.	2006
21559432	The chromatin remodelling complex B-WICH changes the chromatin structure and recruits histone acetyl-transferases to active rRNA genes Böhm, S, Ostlund Farrants, AK, Sadeghifar, F, Mansén, A, Vintermist, A, Percipalle, P, Louvet, E	PLoS ONE	2011
24901984	Glycogen synthase kinase (GSK) 3β phosphorylates and protects nuclear myosin 1c from proteasome-mediated degradation to activate rDNA transcription in early G1 cells Von Euler, A, Borgonovo-Brandter, L, Lamont, D, Visa, N, Percipalle, P, Corcoran, M, Sarshad, AA, Al-Muzzaini, B	PLoS Genet.	2014
24952918	New insight into role of myosin motors for activation of RNA polymerases Percipalle, P, Sarshad, AA	Int Rev Cell Mol Biol	2014
15558034	Nuclear actin and myosin I are required for RNA polymerase I transcription Kahle, M, Dingová, H, de Lanerolle, P, Zhao, J, Zentgraf, H, Iben, S, Hofmann, WA, Kyselá, K, Philimonenko, VV, Grummt, I, Hozák, P	Nat. Cell Biol.	2004
23555303	Nuclear myosin 1c facilitates the chromatin modifications required to activate rRNA gene transcription and cell cycle progression Böhm, S, Sarshad, A, Percipalle, P, Fomproix, N, Sadeghifar, F, Vintermist, A, Louvet, E, Al-Muzzaini, B, Östlund, AK, Mori, R	PLoS Genet.	2013