botG LC cleaves target cell VAMP1

Stable Identifier
Reaction [transition]
Homo sapiens
Related Species
Clostridium botulinum
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Botulinum toxin type G light chain (botG LC), in the cytosol of a target cell, catalyzes the removal of an aminoterminal peptide from vesicle-associated membrane protein 1 (VAMP1). botG LC is a zinc metalloprotease (Schiavo et al. 1994; Yamasaki et al. 1994). VAMP1 is associated with the cytosolic face of the target cell synaptic vesicle and is required for vesicle docking and exocytosis.Its cleavage by botulinum toxin blocks synaptic vesicle fusion with the plasma membrane and neurotransmitter release (Sudhof et al, 1993; Sudhof 2004).
Literature References
PubMed ID Title Journal Year
7527117 Mechanism of action of tetanus and botulinum neurotoxins

Schiavo, G, Montecucco, C

Mol Microbiol 1994
7910017 Botulinum neurotoxin type G proteolyses the Ala81-Ala82 bond of rat synaptobrevin 2

Hayashi, T, Yamasaki, S, Niemann, H, Eklund, M, Szabo, E, Binz, T, Jahn, R, Yamasaki, N

Biochem. Biophys. Res. Commun. 1994
8051110 Botulinum G neurotoxin cleaves VAMP/synaptobrevin at a single Ala-Ala peptide bond

Malizio, C, Polverino de Laureto, P, Trimble, WS, Schiavo, G, Milan, G, Montecucco, C, Johnson, EA, Sugiyama, H

J. Biol. Chem. 1994
15217342 The synaptic vesicle cycle

S├╝dhof, TC

Annu Rev Neurosci 2004
Catalyst Activity

metalloendopeptidase activity of botG LC:Zn2+ [cytosol]

Name Identifier Synonyms
botulism DOID:11976 Botulism (disorder), Intoxication with Clostridium botulinum toxin, Botulism, Infection due to clostridium botulinum, Botulism, Foodborne botulism, botulism, Botulism, Botulism, Botulism, Food poisoning due to Clostridium botulinum, Botulism poisoning
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