MLH1:PMS2 makes single strand incision near 1-2 base mismatch

Stable Identifier
Reaction [transition]
Homo sapiens
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The latent endonuclease activity of MLH1:PMS2 (MutLalpha) is activated by interaction with MSH2:MSH6 and PCNA (Kadyrov et al 2006). MLH1:PMS2 makes a nick in the replicated strand of DNA. As inferred from yeast, more than one MLH1:PMS2 may bind per MSH2:MSH6 (Hombauer et al. 2011). Strand selection of the nick is determined by interaction with PCNA, though the exact mechanism is unknown (Pluciennik et al 2010).
Literature References
PubMed ID Title Journal Year
16143102 Reconstitution of 5'-directed human mismatch repair in a purified system

Gao, Y, Zhang, Y, Li, GM, Yuan, F, Tian, K, Presnell, SR, Gu, L, Tomkinson, AE

Cell 2005
20713735 PCNA function in the activation and strand direction of MutL? endonuclease in mismatch repair

Dzantiev, L, Modrich, P, Iyer, RR, Kadyrov, FA, Pluciennik, A, Constantin, N

Proc. Natl. Acad. Sci. U.S.A. 2010
16188885 Human mismatch repair: reconstitution of a nick-directed bidirectional reaction

Dzantiev, L, Modrich, P, Kadyrov, FA, Constantin, N

J. Biol. Chem. 2005
22118461 Visualization of eukaryotic DNA mismatch repair reveals distinct recognition and repair intermediates

Desai, A, Hombauer, H, Kolodner, RD, Smith, CE, Campbell, CS

Cell 2011
16873062 Endonucleolytic function of MutLalpha in human mismatch repair

Dzantiev, L, Modrich, P, Kadyrov, FA, Constantin, N

Cell 2006
Catalyst Activity

endonuclease activity of MLH1:PMS2:MSH2:MSH6:ATP:PCNA:DNA containing 1-2 base mismatch [nucleoplasm]

Orthologous Events
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