ATAT acetylates microtubules

Stable Identifier
Reaction [transition]
Homo sapiens
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Cilia are enriched in acetylated tubulin, a marker that is associated with stability, and the kinesin motor preferentially travels on acetylated microtubules (Johnson et al, 1998; Reed et al, 2006; Cai et al, 2009). Alpha tubulin acetyltransferase (ATAT), also known as MEC17, has been shown to catalyze the acetylation of alpha tubulin at K40 (Akella et al, 2010; Shida et al, 2010; Montagnac et al, 2013; Taschner et al, 2012). ATAT preferentially acetylates polymerized alpha tubulin and may access the luminal K40 residue through 'breathing' of the microtubules protofilaments (Shida et al, 2010). ATAT was identified as an interacting protein with components of the BBSome, and siRNA knockdown of ATAT delays assembly of the primary cilium (Jin et al, 2010; Shida et al, 2010). BBIP1 is another component of the BBSome that has been shown to affect tubulin acetylation and stability, potentially through its interaction with HDAC6. BBIP1 exerts its effect on microtubule acetylation independently of its role as a component of the BBSome; it may exert its indirect effect by promoting ATAT-mediated acceleration, by counteracting HDAC6-mediated deacetylation, or by another mechanism (Loktev et al, 2008)
Literature References
PubMed ID Title Journal Year
20603001 The conserved Bardet-Biedl syndrome proteins assemble a coat that traffics membrane proteins to cilia

Aguiar, M, Shida, T, Jin, H, Gygi, SP, Nachury, MV, White, SR, Schulz, S, Bazan, JF

Cell 2010
9427680 The axonemal microtubules of the Chlamydomonas flagellum differ in tubulin isoform content

Johnson, KA

J. Cell. Sci. 1998
23071318 Atomic resolution structure of human α-tubulin acetyltransferase bound to acetyl-CoA

Vetter, M, Taschner, M, Lorentzen, E

Proc Natl Acad Sci U S A 2012
24906155 Molecular basis for age-dependent microtubule acetylation by tubulin acetyltransferase

Valenstein, ML, Szyk, A, Roll-Mecak, A, Deaconescu, AM, Kormendi, V, Goodman, B, Grigorieff, N, Ziolkowska, NE, Spector, J

Cell 2014
21068373 The major alpha-tubulin K40 acetyltransferase alphaTAT1 promotes rapid ciliogenesis and efficient mechanosensation

Goodman, MB, Shida, T, Cueva, JG, Nachury, MV, Xu, Z

Proc. Natl. Acad. Sci. U.S.A. 2010
20829795 MEC-17 is an alpha-tubulin acetyltransferase

Gaertig, J, Starostina, NG, Lyons-Abbott, S, Morrissette, NS, Akella, JS, Kipreos, ET, Dougan, ST, Kim, J, Wloga, D

Nature 2010
24097348 ?TAT1 catalyses microtubule acetylation at clathrin-coated pits

Chavrier, P, Montagnac, G, Shida, T, Irondelle, M, Benmerah, A, Nachury, MV, Castro-Castro, A, Olivo-Marin, JC, Franco, M, Meas-Yedid, V

Nature 2013
19823565 Single molecule imaging reveals differences in microtubule track selection between Kinesin motors

Verhey, KJ, McEwen, DP, Martens, JR, Cai, D, Meyhofer, E

PLoS Biol. 2009
17084703 Microtubule acetylation promotes kinesin-1 binding and transport

Gaertig, J, Verhey, KJ, Reed, NA, Jih, GT, Cai, D, Meyhofer, E, Blasius, TL

Curr. Biol. 2006
19081074 A BBSome subunit links ciliogenesis, microtubule stability, and acetylation

Scheetz, TE, Searby, CC, Loktev, AV, Zhang, Q, Sheffield, VC, Nachury, MV, Slusarski, DC, Jackson, PK, Beck, JS, Bazan, JF

Dev. Cell 2008
Catalyst Activity

lysine N-acetyltransferase activity, acting on acetyl phosphate as donor of ATAT [cytosol]

Orthologous Events
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