Reactome | LIG1 bound to APEX1 and PCNA ligates SSB

LIG1 bound to APEX1 and PCNA ligates SSB

Stable Identifier

R-HSA-5651805

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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LIG1 bound to APEX1 and PCNA ligates SSB

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LIG1 (DNA ligase 1) is recruited to the long-patch base excision repair site through its interaction with APEX1, PCNA and RFC. LIG1 ligates the 3' end of the single strand break (SSB), containing the newly synthesized multiple nucleotide repair patch, with the FEN1-processed 5' end of the SSB, thus completing the base excision repair (Klungland and Lindahl 1997, Tomkinson et al. 2001, Ranalli et al. 2002a). The catalytic activity of LIG1 is stimulated by the presence of PCNA-bound RPA (Ranalli et al. 2002b)

Literature References

PubMed ID	Title	Journal	Year
16464020	DNA ligases: structure, reaction mechanism, and function Ellenberger, T, Pascal, JM, Vijayakumar, S, Tomkinson, AE	Chem. Rev.	2006
11698410	Mechanism underlying replication protein a stimulation of DNA ligase I Ranalli, TA, Bambara, RA, DeMott, MS	J. Biol. Chem.	2002
12200445	AP endonuclease 1 coordinates flap endonuclease 1 and DNA ligase I activity in long patch base excision repair. Ranalli, TA, Bambara, RA, Tom, S	J Biol Chem	2002
9214649	Second pathway for completion of human DNA base excision-repair: reconstitution with purified proteins and requirement for DNase IV (FEN1). Lindahl, T, Klungland, A	EMBO J	1997