Reactome | RAD18:UBE2B or RBX1:CUL4:DDB1:DTL ubiquitin ligase complex binds PCNA:POLD,POLE:RPA:RFC associated with damaged dsDNA

RAD18:UBE2B or RBX1:CUL4:DDB1:DTL ubiquitin ligase complex binds PCNA:POLD,POLE:RPA:RFC associated with damaged dsDNA

Stable Identifier

R-HSA-5652005

Type

Reaction [binding]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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RAD18:UBE2B or RBX1:CUL4:DDB1:DTL ubiquitin ligase complex binds PCNA:POLD,POLE:RPA:RFC associated with damaged dsDNA

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The complex of RAD18, an E3 ubiquitin ligase, and UBE2B (RAD6), an E2 ubiquitin-conjugating enzyme, binds the replication complex consisting of PCNA, DNA polymerase complex delta (POLD) or DNA polymerase complex epsilon (POLE), RPA and RFC on damaged dsDNA. RAD18 simultaneously interacts with PCNA, RPA and DNA (Hoege et al. 2002, Notenboom et al. 2007, Davies et al. 2008). The ubiquitin ligase complex RBX1:CUL4:DDB1:DTL can also bind PCNA (Terai et al. 2010).

Literature References

PubMed ID	Title	Journal	Year
18342608	Activation of ubiquitin-dependent DNA damage bypass is mediated by replication protein a Daigaku, Y, Chen, S, Ulrich, HD, Huttner, D, Davies, AA	Mol. Cell	2008
12226657	RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO Moldovan, GL, Hoege, C, Pfander, B, Pyrowolakis, G, Jentsch, S	Nature	2002
17720710	Functional characterization of Rad18 domains for Rad6, ubiquitin, DNA binding and PCNA modification Sixma, TK, Olsen, JV, van Rossum-Fikkert, SE, Notenboom, V, Mann, M, Hibbert, RG	Nucleic Acids Res.	2007
20129063	CRL4(Cdt2) E3 ubiquitin ligase monoubiquitinates PCNA to promote translesion DNA synthesis Abbas, T, Dutta, A, Terai, K, Jazaeri, AA	Mol. Cell	2010