Reactome | POLK and POLZ cooperate in elongation of mispaired primer termini

POLK and POLZ cooperate in elongation of mispaired primer termini

Stable Identifier

R-HSA-5655965

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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POLK and POLZ cooperate in elongation of mispaired primer termini

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DNA polymerase kappa (POLK) can elongate mispaired primer termini generated when an incorrect nucleotide is incorporated opposite a damaged DNA base either by POLK or by another translesion DNA synthesis (TLS) polymerase (Haracska et al. 2002, Carlson et al. 2006). POLK can processively synthesize polynucleotide chains that are usually not more than 20 nucleotides long, generating single base substitutions at a rate of 7/1000 and single base deletions at a rate of 3/1000 (Ohashi et al. 2000). POLK and POLZ can cooperate in the elongation of nucleotides inserted opposite to lesioned bases by POLK (Yoon et al. 2010, Wojtaszek et al. 2012a, Wojtaszek et al. 2012b, Xie et al. 2012, Yoon et al. 2014)

Literature References

PubMed ID	Title	Journal	Year
11006276	Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene Gerlach, VL, Feaver, WJ, Friedberg, EC, Kunkel, TA, Matsuda, T, Ohashi, E, Bebenek, K, Ohmori, H	J. Biol. Chem.	2000
23143872	Structural insights into the assembly of human translesion polymerase complexes Xu, M, Yang, X, Xie, W, Jiang, T	Protein Cell	2012
22859295	Structural basis of Rev1-mediated assembly of a quaternary vertebrate translesion polymerase complex consisting of Rev1, heterodimeric polymerase (Pol) ?, and Pol ? Kim, H, D'Souza, S, Minesinger, B, Lee, CJ, Zhou, P, Walker, GC, Wojtaszek, J, D'Andrea, AD	J. Biol. Chem.	2012
12444249	Role of human DNA polymerase kappa as an extender in translesion synthesis Haracska, L, Prakash, L, Prakash, S	Proc. Natl. Acad. Sci. U.S.A.	2002
22700975	Multifaceted recognition of vertebrate Rev1 by translesion polymerases ? and ? D'Souza, S, Wang, S, Zhou, P, Liu, J, Walker, GC, Wojtaszek, J, Xue, Y	J. Biol. Chem.	2012
17043239	Human DNA polymerase kappa forms nonproductive complexes with matched primer termini but not with mismatched primer termini Washington, MT, Johnson, RE, Carlson, KD, Prakash, L, Prakash, S	Proc. Natl. Acad. Sci. U.S.A.	2006

Participants

Input

Output

Participates

as an event of

Translesion synthesis by POLK (Homo sapiens)

Catalyst Activity

DNA-directed DNA polymerase activity of POLK:REV1:POLZ:MonoUb:K164-PCNA:RPA:RFC:(Tg:A)-DNA Template,(OGUA:A)-DNA Template,(BPDE-G:C)-DNA Template [nucleoplasm]

Physical Entity

POLK:REV1:POLZ:MonoUb:K164-PCNA:RPA:RFC:(Tg:A)-DNA Template,(OGUA:A)-DNA Template,(BPDE-G:C)-DNA Template [nucleoplasm] (Homo sapiens)

Activity

DNA-directed DNA polymerase activity (GO:0003887)

Orthologous Events

POLK and POLZ cooperate in elongation of mispaired primer termini (Bos taurus)

POLK and POLZ cooperate in elongation of mispaired primer termini (Canis familiaris)

POLK and POLZ cooperate in elongation of mispaired primer termini (Drosophila melanogaster)

POLK and POLZ cooperate in elongation of mispaired primer termini (Gallus gallus)

POLK and POLZ cooperate in elongation of mispaired primer termini (Mus musculus)

POLK and POLZ cooperate in elongation of mispaired primer termini (Plasmodium falciparum)

POLK and POLZ cooperate in elongation of mispaired primer termini (Rattus norvegicus)