Reactome | ATR phosphorylates RPA2

ATR phosphorylates RPA2

Stable Identifier

R-HSA-5685156

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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Activated ATR phosphorylates the RPA2 subunit of the RPA complex on serine residue S33. Phosphorylation of RPA2 at S33 likely stimulates additional RPA2 phosphorylation on CDK sites (S23 and S29) and DNA-PKcs (PRKDC) sites (S4, S8 and S21). DNA damage-regulated phosphorylation of RPA2 plays an important role in the progression of homologous recombination-directed repair of DNA double-strand breaks (DSBs) (Anantha et al. 2007, Liu et al. 2012, Murphy et al. 2014)

Literature References

PubMed ID	Title	Journal	Year
17928296	Sequential and synergistic modification of human RPA stimulates chromosomal DNA repair Borowiec, JA, Vassin, VM, Anantha, RW	J Biol Chem	2007
22977173	Distinct roles for DNA-PK, ATM and ATR in RPA phosphorylation and checkpoint activation in response to replication stress Troksa, K, Shrivastav, M, Amerin, C, Nickoloff, JA, Oakley, GG, Liu, S, Ashley, AK, Glanzer, JG, Manthey, K, Opiyo, SO	Nucleic Acids Res.	2012
25113031	Phosphorylated RPA recruits PALB2 to stalled DNA replication forks to facilitate fork recovery Borowiec, JA, Rabinowitsch, AI, Murphy, AK, Schildkraut, CL, Fitzgerald, M, Chowdhury, D, Kim, JH, Ro, T	J. Cell Biol.	2014

Participants

Input

Output

Participates

as an event of

Processing of DNA double-strand break ends (Homo sapiens)

Catalyst Activity

protein serine/threonine kinase activity of ATR:ATRIP:RPA:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm]

Physical Entity

ATR:ATRIP:RPA:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)

Activity

protein serine/threonine kinase activity (GO:0004674)

Orthologous Events

ATR phosphorylates RPA2 (Bos taurus)

ATR phosphorylates RPA2 (Canis familiaris)

ATR phosphorylates RPA2 (Danio rerio)

ATR phosphorylates RPA2 (Gallus gallus)

ATR phosphorylates RPA2 (Mus musculus)

ATR phosphorylates RPA2 (Rattus norvegicus)

ATR phosphorylates RPA2 (Sus scrofa)