Reactome | FEN1 cleaves displaced ssDNA flaps during MMEJ

FEN1 cleaves displaced ssDNA flaps during MMEJ

Stable Identifier

R-HSA-5687664

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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FEN1 cleaves displaced ssDNA flaps during MMEJ

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DNA polymerase theta (POLQ) performs strand displacement DNA synthesis during microhomology-mediated end joining (MMEJ) (Kent et al. 2015), which is expected to result in the formation of displaced 5'-ssDNA flaps. FEN1, a 5'-flap endonuclease, is a necessary participant of MMEJ (Liang et al. 2005, Sharma et al. 2015). By analogy with base excision repair (Klungland and Lindahl 1997, Liu et al. 2005), FEN1 is thought to cleave the 5'-flaps generated by POLQ-mediated DNA strand displacement synthesis during MMEJ, thus enabling the subsequent ligation step.

Literature References

PubMed ID	Title	Journal	Year
15561706	DNA polymerase beta and flap endonuclease 1 enzymatic specificities sustain DNA synthesis for long patch base excision repair Liu, Y, Beard, WA, Wilson, SH, Prasad, R, Hou, EW, Shock, DD	J. Biol. Chem.	2005
25789972	Homology and enzymatic requirements of microhomology-dependent alternative end joining Srivastava, M, Javadekar, SM, Pandey, M, Sharma, S, Kumari, R, Raghavan, SC	Cell Death Dis	2015
16012167	Modulation of DNA end joining by nuclear proteins Li, GC, Shao, C, Tischfield, JA, Deng, L, Chen, Y, Liang, L	J. Biol. Chem.	2005
25643323	Mechanism of microhomology-mediated end-joining promoted by human DNA polymerase ? McDevitt, SM, Chandramouly, G, Kent, T, Ozdemir, AY, Pomerantz, RT	Nat. Struct. Mol. Biol.	2015
9214649	Second pathway for completion of human DNA base excision-repair: reconstitution with purified proteins and requirement for DNase IV (FEN1). Lindahl, T, Klungland, A	EMBO J	1997