NSF ATPase activity dissociates cis-SNARE

Stable Identifier
Reaction [transition]
Homo sapiens
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NSF-dependent hydrolysis of ATP is required to disassociate the cis-SNARE complex, releasing the SNAREs for further rounds of membrane fusion (Mayer et al, 1996; Muller et al, 1999; Muller et al, 2002; Otto et al, 1997; Whiteheart et al, 2004; Yu et al, 1999; Zhao et al, 2012; Shah et al, 2015; reviewed in Sudhof and Rothman, 2009).

Literature References
PubMed ID Title Journal Year
25492864 Three ?SNAP and 10 ATP molecules are used in SNARE complex disassembly by N-ethylmaleimide-sensitive factor (NSF)

Enos, MD, Colbert, KN, Herschlag, D, Shah, N, Weis, WI

J. Biol. Chem. 2015
8620540 Sec18p (NSF)-driven release of Sec17p (alpha-SNAP) can precede docking and fusion of yeast vacuoles

Haas, A, Mayer, A, Wickner, W

Cell 1996
10559959 An NSF function distinct from ATPase-dependent SNARE disassembly is essential for Golgi membrane fusion

Schiavo, G, Rabouille, C, Newman, R, Müller, JM, Shima, DT, Shorter, J, Warren, G, Freemont, P

Nat. Cell Biol. 1999
9177194 Assembly and disassembly of a ternary complex of synaptobrevin, syntaxin, and SNAP-25 in the membrane of synaptic vesicles

Hanson, PI, Jahn, R, Otto, H

Proc. Natl. Acad. Sci. U.S.A. 1997
19164740 Membrane fusion: grappling with SNARE and SM proteins

Südhof, TC, Rothman, JE

Science 2009
21689688 Requirements for the catalytic cycle of the N-ethylmaleimide-Sensitive Factor (NSF)

Whiteheart, SW, Zhao, C, Smith, EC

Biochim. Biophys. Acta 2012
10445031 NSF N-terminal domain crystal structure: models of NSF function

Yu, RC, Jahn, R, Brunger, AT

Mol. Cell 1999
15037235 Multiple binding proteins suggest diverse functions for the N-ethylmaleimide sensitive factor

Matveeva, EA, Whiteheart, SW

J. Struct. Biol. 2004
12070132 Sequential SNARE disassembly and GATE-16-GOS-28 complex assembly mediated by distinct NSF activities drives Golgi membrane fusion

Warren, G, Elazar, Z, Deinhardt, K, Newman, R, Shima, DT, Shorter, J, Muller, JM, Sagiv, Y

J. Cell Biol. 2002
Catalyst Activity

ATP hydrolysis activity of cis-SNARE:NSF hexamer:3xSNAP [endoplasmic reticulum-Golgi intermediate compartment membrane]

Orthologous Events
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