Reactome | ERCC8:DDB1:CUL4:RBX1 ubiquitinates ERCC6 and RNA Pol II

ERCC8:DDB1:CUL4:RBX1 ubiquitinates ERCC6 and RNA Pol II

Stable Identifier

R-HSA-6781867

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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General

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ERCC8:DDB1:CUL4:RBX1 ubiquitinates ERCC6 and RNA Pol II

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The ubiquitin ligase complex ERCC8:DDB1:CUL4:RBX1 may ubiquitinate ERCC6 (CSB) (Groisman et al. 2006) at the later steps of TC-NER and may also be required in the ubiquitination of the RNA Pol II subunit POLR2A in response to damage (Bregman et al. 1996, Lee et al. 2002). Ubiquitination mediated by ERCC8 (CSA) containing ubiquitin ligase complex plays an important role in progression and termination of transcription-coupled nucleotide excision repair (TC-NER), although the mechanistic details are largely unknown.

Literature References

PubMed ID	Title	Journal	Year
16751180	CSA-dependent degradation of CSB by the ubiquitin-proteasome pathway establishes a link between complementation factors of the Cockayne syndrome Kuraoka, I, Kisselev, AF, Tanaka, K, Harel-Bellan, A, Magnaldo, T, Groisman, R, Chevallier, O, Gaye, N, Nakatani, Y	Genes Dev.	2006
11904382	Transcription-coupled and DNA damage-dependent ubiquitination of RNA polymerase II in vitro Lippard, SJ, Sharp, PA, Lee, KB, Wang, D	Proc. Natl. Acad. Sci. U.S.A.	2002
8876179	UV-induced ubiquitination of RNA polymerase II: a novel modification deficient in Cockayne syndrome cells van Gool, AJ, Bregman, DB, Friedberg, EC, Warren, SL, Henning, KA, Halaban, R	Proc. Natl. Acad. Sci. U.S.A.	1996

Participants

Input

Output

Ubiquitinated,hyperphosphorylated RNA Pol II:Damaged DNA template:nascent mRNA hybrid:TFIIH:Ub:ERCC6:ERCC8:DDB1:CUL4:RBX1 [nucleoplasm] (Homo sapiens)

Participates

as an event of

Formation of TC-NER Pre-Incision Complex (Homo sapiens)

Catalyst Activity

ubiquitin protein ligase activity of Hyperphosphorylated RNA Pol II:Damaged DNA template:nascent mRNA hybrid:TFIIH:ERCC6:ERCC8:DDB1:CUL4:RBX1 [nucleoplasm]

Physical Entity

Hyperphosphorylated RNA Pol II:Damaged DNA template:nascent mRNA hybrid:TFIIH:ERCC6:ERCC8:DDB1:CUL4:RBX1 [nucleoplasm] (Homo sapiens)

Activity

ubiquitin protein ligase activity (GO:0061630)

Orthologous Events

ERCC8:DDB1:CUL4:RBX1 ubiquitinates ERCC6 and RNA Pol II (Bos taurus)

ERCC8:DDB1:CUL4:RBX1 ubiquitinates ERCC6 and RNA Pol II (Caenorhabditis elegans)

ERCC8:DDB1:CUL4:RBX1 ubiquitinates ERCC6 and RNA Pol II (Canis familiaris)

ERCC8:DDB1:CUL4:RBX1 ubiquitinates ERCC6 and RNA Pol II (Dictyostelium discoideum)

ERCC8:DDB1:CUL4:RBX1 ubiquitinates ERCC6 and RNA Pol II (Drosophila melanogaster)

ERCC8:DDB1:CUL4:RBX1 ubiquitinates ERCC6 and RNA Pol II (Gallus gallus)

ERCC8:DDB1:CUL4:RBX1 ubiquitinates ERCC6 and RNA Pol II (Mus musculus)