Reactome | Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in TC-NER

Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in TC-NER

Stable Identifier

R-HSA-6782208

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

Locations in the PathwayBrowser

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Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in TC-NER

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In transcription-coupled nucleotide excision repair (TC-NER), as well as in global genome nucleotide excision repair (GG-NER), the DNA synthesis complex (NER post-incision complex) consisting of PCNA, RPA, RFC and polymerase delta (POLD) or epsilon (POLE) complexes performs DNA repair synthesis after the damaged DNA strand is incised 5' to the lesion by the endonuclease complex ERCC1:ERCC4 (ERCC1:XPF) and 3' to the lesion by the endonuclease XPG (ERCC5). Depending on damage-induced PCNA monoubiquitination, DNA polymerase kappa (POLK) may also be involved in gap-filling DNA synthesis during nucleotide excision repair (NER) (Balajee et al. 1998, Staresincic et al. 2009, Ogi et al. 2010, Overmeer et al. 2011).

Literature References

PubMed ID	Title	Journal	Year
20227374	Three DNA polymerases, recruited by different mechanisms, carry out NER repair synthesis in human cells Ogi, T, Limsirichaikul, S, Overmeer, RM, Volker, M, Takenaka, K, Cloney, R, Nakazawa, Y, Niimi, A, Miki, Y, Jaspers, NG, Mullenders, LH, Yamashita, S, Fousteri, M, Lehmann, AR	Mol. Cell	2010
9875289	Efficient PCNA complex formation is dependent upon both transcription coupled repair and genome overall repair Balajee, AS, May, A, Dianova, I, Bohr, VA	Mutat. Res.	1998
21282463	Replication protein A safeguards genome integrity by controlling NER incision events Overmeer, RM, Moser, J, Volker, M, Kool, H, Tomkinson, AE, van Zeeland, AA, Mullenders, LH, Fousteri, M	J. Cell Biol.	2011
19279666	Coordination of dual incision and repair synthesis in human nucleotide excision repair Staresincic, L, Fagbemi, AF, Enzlin, JH, Gourdin, AM, Wijgers, N, Dunand-Sauthier, I, Giglia-Mari, G, Clarkson, SG, Vermeulen, W, Schärer, OD	EMBO J.	2009

Participants

Input

Output

Participates

as an event of

Gap-filling DNA repair synthesis and ligation in TC-NER (Homo sapiens)

Catalyst Activity

DNA-directed DNA polymerase activity of TC-NER post-incision complex:incised DNA without lesion:trimmed nascent mRNA:PCNA:(PCNA:POLD,POLE),(MonoUb:K164-PCNA:POLK):RPA:RFC [nucleoplasm]

Physical Entity

TC-NER post-incision complex:incised DNA without lesion:trimmed nascent mRNA:PCNA:(PCNA:POLD,POLE),(MonoUb:K164-PCNA:POLK):RPA:RFC [nucleoplasm] (Homo sapiens)

Activity

DNA-directed DNA polymerase activity (GO:0003887)

Orthologous Events

Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in TC-NER (Bos taurus)

Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in TC-NER (Caenorhabditis elegans)

Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in TC-NER (Canis familiaris)

Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in TC-NER (Danio rerio)

Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in TC-NER (Dictyostelium discoideum)

Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in TC-NER (Drosophila melanogaster)

Repair DNA synthesis of ~27-30 bases long patch by POLD, POLE or POLK in TC-NER (Gallus gallus)